Molecular and mutational analysis of a DNA region separating two methylotrophy gene clusters in Methylobacterium extorquens AM1.

Microbiology
Ludmila Chistoserdova, Mary E Lidstrom

Abstract

A region of 14.2 kb has been analysed that is a part of a locus on the Methylobacterium extorquens AM1 chromosome containing a number of genes involved in one-carbon (C1) metabolism, including serine cycle genes, pqq genes, regulatory methanol oxidation genes and the gene for N5,N10-methylene tetrahydrofolate dehydrogenase (mtdA). Fifteen new ORFs have been identified within the new region, and their sequences suggest that they encode the following polypeptides: the C-terminal part of phosphoenolpyruvate carboxylase, malyl-CoA lyase, polypeptides of 9.4 and 31 kDa of unknown function, three putative subunits of an ABC-type transporter, two polypeptides similar to the products of mxaF and mxaJ from M. extorquens AM1 and other methylotrophs, a cytochrome c, three enzymes of folate metabolism, and polypeptides of 13 and 20.5 kDa with no homologues in the protein database. Ten insertion mutations have been generated in the region to determine if the newly identified genes are associated with C1 metabolism. A mutation in mclA, encoding malyl-CoA lyase, resulted in a C1-minus phenotype, while mutations in the other genes all showed a C1-plus phenotype. It was not possible to obtain null mutants in a putative folate metabolism gene, f...Continue Reading

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Citations

Jul 16, 2008·Applied and Environmental Microbiology·Talia JewellDouglas C Nelson
Aug 30, 2011·Journal of Bacteriology·Elizabeth SkovranMary E Lidstrom
May 6, 2003·Journal of Bacteriology·Ludmila ChistoserdovaMary E Lidstrom
Aug 7, 2012·Biochemistry. Biokhimii︠a︡·A R GvozdevR I Gvozdev
May 13, 2014·Applied Microbiology and Biotechnology·Jan T KeltjensHuub J M Op den Camp
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Nathanaël DelmotteJulia A Vorholt
Oct 21, 2005·Journal of Bacteriology·Marina G Kalyuzhnaya, Mary E Lidstrom
Nov 12, 2011·Mikrobiologiia·D N FedorovIu A Trotsenko
May 7, 2015·Environmental Microbiology·Martin TaubertJ Colin Murrell
Nov 29, 2005·Environmental Microbiology·Marina G KalyuzhnayaLudmila Chistoserdova
Apr 9, 2008·Environmental Microbiology·Stephen J GiovannoniMichael S Rappé
Mar 30, 2011·Environmental Microbiology·Ludmila Chistoserdova
Aug 14, 2013·Metabolic Engineering·Samuel E MainguetJames C Liao
Jun 9, 2015·Applied Microbiology and Biotechnology·Ludmila Chistoserdova
Nov 30, 2014·Applied Microbiology and Biotechnology·Andrea M OchsnerJulia A Vorholt
Jul 1, 2016·World Journal of Microbiology & Biotechnology·Ludmila Chistoserdova
Jan 7, 2018·Applied and Environmental Microbiology·Jeremy D SemrauSukhwan Yoon
Dec 3, 2016·International Journal of Systematic and Evolutionary Microbiology·Haoxin LvAkio Tani
Dec 21, 2014·Applied and Environmental Microbiology·Ming L WuLaura van Niftrik
Sep 27, 2018·IUBMB Life·Giovanna De SimonePaolo Ascenzi
Oct 13, 2019·Applied and Environmental Microbiology·Carl-Eric WegnerKirsten Küsel
Mar 29, 2018·MBio·Yue ZhengLudmila Chistoserdova
Jan 5, 2008·Microbiology·Shondelle M WilsonTimothy J Donohue
Jan 18, 2019·Molecular Microbiology·Andrea M OchsnerJulia A Vorholt

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