Molecular and pharmacological characterization of the Chelicerata pyrokinin receptor from the southern cattle tick, Rhipicephalus (Boophilus) microplus

Insect Biochemistry and Molecular Biology
Yunlong YangP V Pietrantonio

Abstract

We identified the first pyrokinin receptor (Rhimi-PKR) in Chelicerata and analyzed structure-activity relationships of cognate ligand neuropeptides and their analogs. Based on comparative and phylogenetic analyses, this receptor, which we cloned from larvae of the cattle tick Rhipicephalus microplus (Acari: Ixodidae), is the ortholog of the insect pyrokinin (PK)/pheromone biosynthesis activating neuropeptide (PBAN)/diapause hormone (DH) neuropeptide family receptor. Rhimi-PKR functional analyses using calcium bioluminescence were performed with a developed stable recombinant CHO-K1 cell line. Rhimi-PKR was activated by four endogenous PKs from the Lyme disease vector, the tick Ixodes scapularis (EC50s range: 85.4 nM-546 nM), and weakly by another tick PRX-amide peptide, periviscerokinin (PVK) (EC50 = 24.5 μM). PK analogs with substitutions of leucine, isoleucine or valine at the C-terminus for three tick PK peptides, Ixosc-PK1, Ixosc-PK2, and Ixosc-PK3, retained their potency on Rhimi-PKR. Therefore, Rhimi-PKR is less selective and substantially more tolerant than insect PK receptors of C-terminal substitutions of leucine to isoleucine or valine, a key structural feature that serves to distinguish insect PK from PVK/CAP2b recep...Continue Reading

References

Apr 18, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Laura KeanJulian A T Dow
Dec 3, 2002·Biochemical and Biophysical Research Communications·Annette IversenCornelis J P Grimmelikhuijzen
Jul 31, 2003·Proceedings of the National Academy of Sciences of the United States of America·Man-Yeon ChoiRussell Jurenka
Aug 2, 2005·Biochemical and Biophysical Research Communications·Giuseppe CazzamaliCornelis J P Grimmelikhuijzen
Apr 8, 2006·Biochemical and Biophysical Research Communications·Takashi HommaToshinobu Yaginuma
Oct 27, 2006·Journal of Neurophysiology·Shari R SaidemanMichael P Nusbaum
Aug 22, 2007·Biochemical and Biophysical Research Communications·Stine S OlsenFrank Hauser
May 23, 2008·General and Comparative Endocrinology·Ada Rafaeli
Nov 6, 2008·General and Comparative Endocrinology·Suparna Taneja-BageshwarRonald J Nachman
Jan 10, 2009·Biochemical and Biophysical Research Communications·Marleen LindemansLiliane Schoofs
Feb 3, 2009·Bioorganic & Medicinal Chemistry·Jürgen Scherkenbeck, Tino Zdobinsky
Jun 23, 2009·Journal of Proteomics·Susanne NeupertRonald J Nachman
Dec 3, 2009·Peptides·Ladislav RollerDusan Zitnan
Aug 17, 2010·Molecular and Cellular Endocrinology·Rainer StrotmannTorsten Schöneberg
Oct 5, 2010·General and Comparative Endocrinology·Andrew E ChristiePetra H Lenz
Nov 9, 2010·Parasites & Vectors·Danett K BrakeAdalberto A Pérez de León
Sep 24, 2011·Proceedings of the National Academy of Sciences of the United States of America·Qirui ZhangDavid L Denlinger
May 29, 2012·Insect Biochemistry and Molecular Biology·Jennie S Garbutt, Stuart E Reynolds
Dec 12, 2012·Frontiers in Endocrinology·Jelle CaersLiliane Schoofs
Dec 12, 2012·PloS One·Man-Yeon Choi, Robert K Vander Meer
Jun 4, 2013·Molecular and Cellular Endocrinology·Man-Yeon ChoiRobert K Vander Meer

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