Molecular and Phenotypic Study of Carbapenem Resistant E. coli

Clinical Laboratory
Firyal N Aziz

Abstract

Carbapenem resistance is an emerging problem. The aim of the present study was to determine the prevalence of carbapenem resistance and ESBLs among clinical isolates of E. coli by phenotypic methods and to study the molecular bases of the resistance by polymerase chain reaction (PCR). The study was carried on 153 non repetitive E. coli strains collected from different clinical samples from Baghdad hospitals. The strains were isolated according to standard microbiological procedures and identified by Gram stain and biochemical reactions. E. coli strains were subjected to antimicrobial susceptibility tests by the disc diffusion method, carbapenem screening, ESBL screening tests, and molecular studies for genes responsible for such resistance. The study was carried out on 153 non repetitive E. coli. Detection of ESBLs by the double disc method reveals that 63 isolates (41.2%) of isolated E. coli had ESBL activity. Carbapenem resistance among E. coli reveals that 30 isolates (19.6%) had both metallo-β-lactamase and carbapenemase activity as detected by double disc synergy test and boronic acid disc. Among 63 isolates of E. coli with positive double disc tests for ESBL resistance, the most frequent gene detected by PCR was blaTEM (5...Continue Reading

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