Molecular and ultrastructural characterization of porcine hippurate-negative Brachyspira pilosicoli

Journal of Clinical Microbiology
M FossiS Pelkonen

Abstract

Brachyspira pilosicoli, the causative agent of porcine intestinal spirochetosis, usually has hippurate-cleaving capacity. We have regularly isolated hippurate-negative B. pilosicoli from cases of porcine diarrhea. In this study, we show that these biochemically atypical B. pilosicoli isolates can be classified as B. pilosicoli. 16S ribosomal DNA was partially sequenced from eight hippurate-negative and two hippurate-positive B. pilosicoli-like isolates from seven herds. The differences in nucleotide sequence with B. pilosicoli P43/6/78 type strain were not associated with hippurate cleavage. In 877 bp, the hippurate-negative isolates had a similarity of 98.63 to 100% to the type strain, with the corresponding figures for the two hippurate-positive isolates being 98.86 and 100%. The nucleotide sequences of hippurate-positive isolates were identical to the respective sequences of hippurate-negative isolates from one herd. The DNA macrorestriction patterns of a total of 20 hippurate-negative and -positive B. pilosicoli isolates were diverse, and no clustering in conjunction with the hippurate reaction was found. In two herds, hippurate-positive and -negative B. pilosicoli isolates had a common macrorestriction pattern. The ultrast...Continue Reading

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Nov 5, 2003·Epidemiology and Infection·M FossiiS Pelkonen

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Citations

Dec 19, 2013·PloS One·Francisco Javier Martínez-LoboPedro Rubio
Dec 22, 2007·Journal of Microbiological Methods·Claes FellströmAnna Aspán
Nov 19, 2013·Letters in Applied Microbiology·S ProhaskaH Huber
Aug 24, 2012·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Yogesh ChanderConnie J Gebhart

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