Molecular approach to the identification of fish in the South China Sea.

PloS One
Junbin Zhang, Robert Hanner

Abstract

DNA barcoding is one means of establishing a rapid, accurate, and cost-effective system for the identification of species. It involves the use of short, standard gene targets to create sequence profiles of known species against sequences of unknowns that can be matched and subsequently identified. The Fish Barcode of Life (FISH-BOL) campaign has the primary goal of gathering DNA barcode records for all the world's fish species. As a contribution to FISH-BOL, we examined the degree to which DNA barcoding can discriminate marine fishes from the South China Sea. DNA barcodes of cytochrome oxidase subunit I (COI) were characterized using 1336 specimens that belong to 242 species fishes from the South China Sea. All specimen provenance data (including digital specimen images and geospatial coordinates of collection localities) and collateral sequence information were assembled using Barcode of Life Data System (BOLD; www.barcodinglife.org). Small intraspecific and large interspecific differences create distinct genetic boundaries among most species. In addition, the efficiency of two mitochondrial genes, 16S rRNA (16S) and cytochrome b (cytb), and one nuclear ribosomal gene, 18S rRNA (18S), was also evaluated for a few select groups...Continue Reading

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Citations

Mar 19, 2013·BMC Genetics·Luiz H G PereiraClaudio Oliveira
Nov 28, 2013·Mitochondrial DNA·Jonas P Quilang, Shiny Cathlynne S Yu
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Nov 3, 2016·Molecular Ecology Resources·Chia-Hao ChangPai-Lei Lin
May 13, 2021·Ecology and Evolution·Md Sagir AhmedZarif Hossain

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Methods Mentioned

BETA
PCR

Software Mentioned

SeqScape
BOLD
SPSS
Barcode of Life Data System ( BOLD )
MEGA

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