Molecular architecture of axonemal microtubule doublets revealed by cryo-electron tomography

Nature
Haixin Sui, Kenneth H Downing

Abstract

The axoneme, which forms the core of eukaryotic flagella and cilia, is one of the largest macromolecular machines, with a structure that is largely conserved from protists to mammals. Microtubule doublets are structural components of axonemes that contain a number of proteins besides tubulin, and are usually found in arrays of nine doublets arranged around two singlet microtubules. Coordinated sliding of adjacent doublets, which involves a host of other proteins in the axoneme, produces periodic beating movements of the axoneme. We have obtained a three-dimensional density map of intact microtubule doublets using cryo-electron tomography and image averaging. Our map, with a resolution of about 3 nm, provides insights into locations of particular proteins within the doublets and the structural features of the doublets that define their mechanical properties. We identify likely candidates for several of these non-tubulin components of the doublets. This work offers insight on how tubulin protofilaments and accessory proteins attach together to form the doublets and provides a structural basis for understanding doublet function in axonemes.

References

Sep 1, 1992·The Journal of Cell Biology·D N MastronardeM E Porter
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J M NorranderR W Linck
Jan 1, 1989·Cell Motility and the Cytoskeleton·W Steffen, R W Linck
Apr 1, 1987·The Journal of Cell Biology·R W Linck, R E Stephens
Jan 1, 1982·Progress in Clinical and Biological Research·R W LinckG L Langevin
Jan 1, 1993·Cell Motility and the Cytoskeleton·H H Bozkurt, D M Woolley
Dec 31, 2002·The EMBO Journal·Santwana KarLinda A Amos
Dec 9, 2003·Protist·Richard W Linck, Jan M Norrander
Nov 30, 2004·Biology of the Cell·Charles B Lindemann
Sep 15, 2005·International Review of Cytology·Linda Kohl, Philippe Bastin

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Citations

Mar 24, 2011·Nature Reviews. Molecular Cell Biology·Hiroaki Ishikawa, Wallace F Marshall
Oct 17, 2008·Nature Reviews. Microbiology·Michael L GingerPaul G McKean
Jan 19, 2012·Biologie aujourd'hui·Cathy Fisch, Pascale Dupuis-Williams
May 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Koji IkegamiMitsutoshi Setou
Jun 22, 2011·Proceedings of the National Academy of Sciences of the United States of America·Alexey Y KoyfmanWah Chiu
Sep 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Daniela NicastroRichard W Linck
Jun 15, 2007·The Journal of Experimental Medicine·Marek CyrklaffFriedrich Frischknecht
May 19, 2011·Molecular Human Reproduction·Kazuo Inaba
Nov 28, 2007·Zhurnal evoliutsionnoĭ biokhimii i fiziologii·V L SviderskiĭS I Plotnikova
Jul 7, 2009·Annual Review of Microbiology·Katherine S RalstonKent L Hill
Aug 2, 2008·Genome Biology·Linda A Amos
Jul 23, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Mark Winey, Eileen O'Toole
Jul 8, 2009·Eukaryotic Cell·Drashti DaveJacek Gaertig
Sep 7, 2006·The Journal of Cell Biology·Boyan K GarvalovMarek Cyrklaff
Dec 17, 2009·The Journal of Cell Biology·Thomas HeuserDaniela Nicastro
Aug 12, 2009·The Journal of Cell Biology·Khanh Huy BuiTakashi Ishikawa
Jan 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Shuiqiao YuanWei Yan
Jun 5, 2012·Micron : the International Research and Review Journal for Microscopy·Jose-Jesus Fernandez
Apr 13, 2010·Journal of Theoretical Biology·Christian CibertGuy Bonnet
Jun 30, 2009·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Roman I Koning, Abraham J Koster
Feb 3, 2009·Current Opinion in Cell Biology·Andreas Hoenger, J Richard McIntosh
Oct 10, 2007·Journal of Structural Biology·Roman I KoningA Mieke Mommaas
May 29, 2007·Tissue & Cell·Romano DallaiPietro Lupetti
Mar 28, 2007·Current Opinion in Structural Biology·Kenneth H Downing, Haixin Sui
Mar 30, 2007·Journal of Molecular Biology·Takashi IshikawaKazuhiro Oiwa
Mar 17, 2007·Cell Motility and the Cytoskeleton·Richard W Linck, Raymond E Stephens
Jul 27, 2007·Cell Motility and the Cytoskeleton·Kazuho IkedaRitsu Kamiya
Jul 23, 2008·Angewandte Chemie·Pei YuanChengzhong Yu
Apr 19, 2008·Cell Motility and the Cytoskeleton·David P LorchAlan J Hunt
Jul 7, 2011·Biology of the Cell·Cathy Fisch, Pascale Dupuis-Williams
Feb 2, 2012·The EMBO Journal·Swadhin C JanaMónica Bettencourt-Dias
Jul 27, 2015·Journal of Structural Biology·Gregory P KishchenkoHaixin Sui
Feb 18, 2014·Current Opinion in Cell Biology·Swadhin Chandra JanaMónica Bettencourt-Dias
Jun 12, 2012·Journal of Molecular Biology·Naoko MizunoEsben Lorentzen

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