Molecular architecture of fungal cell walls revealed by solid-state NMR

Nature Communications
Xue KangTuo Wang

Abstract

The high mortality of invasive fungal infections, and the limited number and inefficacy of antifungals necessitate the development of new agents with novel mechanisms and targets. The fungal cell wall is a promising target as it contains polysaccharides absent in humans, however, its molecular structure remains elusive. Here we report the architecture of the cell walls in the pathogenic fungus Aspergillus fumigatus. Solid-state NMR spectroscopy, assisted by dynamic nuclear polarization and glycosyl linkage analysis, reveals that chitin and α-1,3-glucan build a hydrophobic scaffold that is surrounded by a hydrated matrix of diversely linked β-glucans and capped by a dynamic layer of glycoproteins and α-1,3-glucan. The two-domain distribution of α-1,3-glucans signifies the dual functions of this molecule: contributing to cell wall rigidity and fungal virulence. This study provides a high-resolution model of fungal cell walls and serves as the basis for assessing drug response to promote the development of wall-targeted antifungals.

References

Jan 20, 1995·The Journal of Biological Chemistry·R KollárE Cabib
Nov 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·V T Andriole
Sep 1, 1996·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D W Denning
Feb 1, 1997·Biochemical Society Transactions·T FontaineJ P Latgé
Jun 13, 1998·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D W Denning
Jul 31, 1998·Journal of Bacteriology·P N Lipke, R Ovalle
Apr 9, 1999·Clinical Microbiology Reviews·J P Latgé
Feb 15, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·S J LinS M Teutsch
Aug 1, 2002·Proceedings of the National Academy of Sciences of the United States of America·Chad M RienstraRobert G Griffin
Sep 5, 2002·Journal of the American Chemical Society·Christopher P JaroniecRobert G Griffin
Jun 26, 2003·Trends in Microbiology·Frank C OddsNeil A R Gow
May 26, 2004·Methods : a Companion to Methods in Enzymology·Pilar Pérez, Juan C Ribas
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Guillaume Lesage, Howard Bussey
Aug 24, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Shaun M Bowman, Stephen J Free
Oct 3, 2006·International Journal of Biological Macromolecules·Toshifumi YuiSachio Hayashi
Jan 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Chad A RappleyeWilliam E Goldman
Sep 15, 2007·Molecular Microbiology·Jean-Paul Latgé
Dec 10, 2008·Journal of the American Chemical Society·Christian AderMarc Baldus
Apr 2, 2009·Biomacromolecules·Pawel SikorskiMasahisa Wada
Apr 2, 2009·Journal of the American Chemical Society·Józef R LewandowskiRobert G Griffin
Aug 29, 2009·Nature·Vishukumar AimaniandaJean-Paul Latgé
Apr 30, 2011·Future Microbiology·David S Perlin
Nov 2, 2011·Journal of the American Chemical Society·Arne H LindenHartmut Oschkinat
Dec 17, 2011·Applied Microbiology and Biotechnology·Helene C Eisenman, Arturo Casadevall
Dec 21, 2012·Science Translational Medicine·Gordon D BrownTheodore C White
Jan 25, 2013·Frontiers in Microbiology·Thorsten HeinekampAxel A Brakhage
Mar 23, 2013·Accounts of Chemical Research·Aaron J RossiniLyndon Emsley
Apr 20, 2013·Accounts of Chemical Research·Qing Zhe NiRobert G Griffin
Sep 26, 2013·Proceedings of the National Academy of Sciences of the United States of America·Tuo WangMei Hong
Oct 7, 2014·Journal of Biomolecular NMR·Eline J KoersMarc Baldus
Oct 30, 2014·Frontiers in Cellular and Infection Microbiology·Eliana Barreto-Bergter, Rodrigo T Figueiredo

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Citations

Feb 29, 2020·Cells·Yordanis Pérez-LlanoMaría Del Rayo Sánchez-Carbente
Mar 4, 2020·Journal of Biomolecular NMR·Xue KangTuo Wang
May 8, 2020·Biochemical Society Transactions·Arnab ChakrabortyTuo Wang
Nov 15, 2019·Clinical Microbiology Reviews·Jean-Paul Latgé, Georgios Chamilos
Nov 2, 2019·Nature Communications·Oliver M TerrettPaul Dupree
Apr 24, 2020·Biotechnology for Biofuels·Gijs van ErvenMirjam A Kabel
May 16, 2020·Frontiers in Bioengineering and Biotechnology·Moira GiovannoniBenedetta Mattei
Apr 23, 2020·Frontiers in Microbiology·Ralph GötzUlrich Terpitz
May 14, 2020·AIDS Research and Therapy·Jing OuyangJean-Pierre Routy
Dec 16, 2018·Cellular Microbiology·Isabel ValsecchiAnne Beauvais
Mar 17, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Vikram MehrajUNKNOWN Montreal Primary HIV Infection Study and Canadian HIV and Aging Cohort Study Groups
Jul 8, 2020·International Journal of Molecular Sciences·Sarah A OverallAlexander B Barnes
Jul 12, 2020·Applied Microbiology and Biotechnology·Puangpen LimsakulKhanok Ratanakhanokchai
Jul 14, 2020·Frontiers in Cellular and Infection Microbiology·Muxing LiuPing Wang
Sep 16, 2020·Journal of Experimental Botany·Alan WankeAlga Zuccaro
Jul 31, 2019·Journal of Magnetic Resonance·Gregg Siegal, Philipp Selenko
Jan 8, 2021·Environmental Science and Pollution Research International·Vinay Kumar, Shiv Kumar Dwivedi
Feb 21, 2021·Advanced Drug Delivery Reviews·Pyae PhyoYongchao Su
Apr 7, 2020·Solid State Nuclear Magnetic Resonance·Wancheng ZhaoTuo Wang
Sep 9, 2020·Solid State Nuclear Magnetic Resonance·John E KellyRuth E Stark
Nov 14, 2020·Carbohydrate Polymers·Mikhail V TsurkanHermann Ehrlich
Feb 8, 2020·Biochimica Et Biophysica Acta. General Subjects·Takat B RawalLoukas Petridis
May 4, 2021·Environmental Microbiology·Baojun Wu, Murray P Cox
May 18, 2021·Current Opinion in Biotechnology·Ena Šečić, Karl-Heinz Kogel
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Olga TsivilevaAnastasiya Ivanova

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Methods Mentioned

BETA
NMR
ssNMR
X-ray
acetylation

Software Mentioned

INADEQUATE

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An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

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Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

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