Molecular assembly and structure of the bacteriophage T4 tail

Biophysics Reviews
F ArisakaM G Rossmann

Abstract

The tail of bacteriophage T4 undergoes large structural changes upon infection while delivering the phage genome into the host cell. The baseplate is located at the distal end of the contractile tail and plays a central role in transmitting the signal to the tail sheath that the tailfibers have been adsorbed by a host bacterium. This then triggers the sheath contraction. In order to understand the mechanism of assembly and conformational changes of the baseplate upon infection, we have determined the structure of an in vitro assembled baseplate through the three-dimensional reconstruction of cryo-electron microscopy images to a resolution of 3.8 Å from electron micrographs. The atomic structure was fitted to the baseplate structure before and after sheath contraction in order to elucidate the conformational changes that occur after bacteriophage T4 has attached itself to a cell surface. The structure was also used to investigate the protease digestion of the assembly intermediates and the mutation sites of the tail genes, resulting in a number of phenotypes.

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Citations

Jun 29, 2017·ACS Synthetic Biology·Pan TaoVenigalla Rao
Oct 8, 2017·Biophysics Reviews·Shigeki Takeda
Nov 18, 2017·Biophysics Reviews·Hiroshi Inaba, Takafumi Ueno
Dec 16, 2017·Biophysics Reviews·Philip SerwerWen Jiang
Dec 16, 2017·Biophysics Reviews·Koichi Kato, Tadashi Satoh
May 17, 2017·Biophysics Reviews·Cris Dos Remedios
Dec 6, 2017·Biophysics Reviews·Tadayuki Ogawa, Nobutaka Hirokawa
Oct 17, 2018·Nature Microbiology·Yassine CherrakEric Durand
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Jun 22, 2019·Annual Review of Microbiology·Jing WangMarek Basler

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