Molecular asymmetry in alkaline phosphatase of Escherichia coli

Archives of Biochemistry and Biophysics
O P MalhotraD K Srivastava

Abstract

Thermal inactivation of alkaline phosphatase of Escherichia coli has been studied at different temperatures (45 to 70 degrees C) and pHs (7.5, 9.0, and 10.0) for the commercial, buffer-dialyzed (pH 9.0) and EDTA-dialyzed (pH 9.0) enzymes. In each case, the inactivation exhibits biphasic kinetics consistent with the rate equation, (formula; see text) where A0 and A are activities at time zero and t, and k1 and k2 are first-order rate constants for the fast and slow phase, respectively. Values of k1 and k2 change independently with temperature, pH, and pretreatment (dialysis) of the enzyme. Time course of inactivation of the enzyme with excess EDTA and effect of Zn2+ ion concentration on the activity of EDTA-dialyzed enzyme have been investigated. The data suggest that the dimeric enzyme protein has two types of catalytic sites which have equal catalytic efficiency (or specific activity) but differ in several other properties. Structural implications of these results have been discussed.

References

Feb 22, 1977·Biochemistry·W F BosronB L Vallee
Jun 1, 1974·Archives of Biochemistry and Biophysics·J E NorneB Lindman
Mar 1, 1974·CRC Critical Reviews in Biochemistry·F SeydouxS A Bernhard
Feb 1, 1974·Biophysical Chemistry·F Seydoux, S Bernhard
Mar 15, 1973·Journal of Molecular Biology·J R Knox, H W Wyckoff
Dec 20, 1967·Journal of the American Chemical Society·S H Ko, F J Kézdy
Nov 1, 1967·Biochemistry·J A Reynolds, M J Schlesinger
Nov 1, 1971·The Biochemical Journal·S E Halford
Apr 1, 1969·European Journal of Biochemistry·C LazdunskiM Lazdunski
Jan 28, 1971·Journal of Molecular Biology·R A MacQuarrie, S A Bernhard
Apr 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·A Levitzki, D E Koshland
Jun 24, 1980·Biochemistry·M CociveraI B Wilson
Apr 1, 1963·Journal of Molecular Biology·F ROTHMAN, R BYRNE
May 25, 1962·Biochemistry·D J PLOCKEB L VALLEE

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Citations

Nov 19, 2003·European Journal of Biochemistry·Stjepan Orhanović, Maja Pavela-Vrancic
Jan 15, 1995·European Journal of Biochemistry·O P MalhotraA M Kayastha
Dec 1, 1984·Archives of Biochemistry and Biophysics·O P MalhotraU N Dwivedi
Feb 1, 1985·Archives of Biochemistry and Biophysics·O P Malhotra, Srinivasan
Nov 1, 1991·Archives of Biochemistry and Biophysics·A A IglesiasC S Andreo

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