Molecular-based approaches to characterize coastal microbial community and their potential relation to the trophic state of Red Sea

Scientific Reports
Mohd Ikram AnsariPei-Ying Hong

Abstract

Molecular-based approaches were used to characterize the coastal microbiota and to elucidate the trophic state of Red Sea. Nutrient content and enterococci numbers were monitored, and used to correlate with the abundance of microbial markers. Microbial source tracking revealed the presence of >1 human-associated Bacteroides spp. at some of the near-shore sampling sites and at a heavily frequented beach. Water samples collected from the beaches had occasional exceedances in enterococci numbers, higher total organic carbon (TOC, 1.48-2.18 mg/L) and nitrogen (TN, 0.15-0.27 mg/L) than that detected in the near-shore waters. Enterococci abundances obtained from next-generation sequencing did not correlate well with the cultured enterococci numbers. The abundance of certain genera, for example Arcobacter, Pseudomonas and unclassified Campylobacterales, was observed to exhibit slight correlation with TOC and TN. Low abundance of functional genes accounting for up to 41 copies/L of each Pseudomonas aeruginosa and Campylobacter coli were detected. Arcobacter butzleri was also detected in abundance ranging from 111 to 238 copies/L. Operational taxonomic units (OTUs) associated with cyanobacteria, Prochlorococcus, Ostreococcus spp. and Gr...Continue Reading

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Citations

Oct 9, 2015·International Journal of Environmental Research and Public Health·Dhafer AlsalahPei-Ying Hong
Jan 8, 2016·FEMS Microbiology Ecology·J K PearmanS Carvalho
Jun 22, 2016·Antimicrobial Agents and Chemotherapy·David Mantilla-CalderonPei-Ying Hong
Dec 13, 2017·Environmental Science and Pollution Research International·Anwesha Ghosh, Punyasloke Bhadury
May 28, 2021·Frontiers in Microbiology·Abbrar LabbanXosé Anxelu G Morán

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Datasets Mentioned

BETA
PRJEB5835
KJ571203-KJ571216

Methods Mentioned

BETA
PCR
Flow cytometry

Software Mentioned

Primer
AutoCAD
RDP Infernal Aligner
BLASTN
RDP
Perl Script
CD
Hit
RDP Classifier
Greengenes

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