PMID: 9541638Apr 29, 1998Paper

Molecular bases of hprt mutations in malathion-treated human T-lymphocytes

Mutation Research
J M PluthR J Albertini

Abstract

Recently, we reported that 6 of 84 (7.1%) hprt mutants arising in in vitro malathion-treated human T-lymphocytes were characterized by specific genomic deletions in a 125-bp region of exon 3 (Pluth et al., Cancer Research 56 (1996) 2393-2399. We have now extended study to determine whether additional differences in molecular spectrum at a basepair level exist between control and malathion-treated mutations, and investigated whether there is evidence to support the hypothesis that malathion is an alkylating agent. We analyzed 101 hprt mutants (24 from control and 77 from treated cultures) isolated form six in vitro malathion exposures of T-lymphocytes from four healthy male donors. Analysis consisted of: Southern blotting, genomic multiplex PCR, genomic DNA sequencing and reverse transcription of PCR amplification (RT/PCR) and sequencing of the cDNA product. Mutations at several basepair sites were frequent after malathion exposure and were isolated from treated cells from at least two different individuals. Using a human hprt mutation database for comparison, the frequency of mutations at one of these sites (basepair 134) was found to be significantly elevated in the malathion-treated cell (p < 0.0005). Hprt mutations in malath...Continue Reading

References

Jan 1, 1992·Environmental and Molecular Mutagenesis·N F CarielloT R Skopek
Apr 1, 1990·Genomics·A EdwardsW Ansorge
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·Y YanagiT W Mak
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May 5, 1993·Journal of Molecular Biology·N F Cariello, T R Skopek

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Citations

Sep 15, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Eduardo CasasMiguel Betancourt
Apr 25, 2007·International Journal of Toxicology·Zayil SalazarHumberto González-Márquez
Mar 13, 2003·World Journal of Gastroenterology : WJG·Sheng-Xue LiuYong Liu
Aug 14, 2020·Ciência & saúde coletiva·Priscilla Luna BastosIdê Gomes Dantas Gurgel

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