Molecular basis for ADP-induced platelet activation. II. The P2Y1 receptor mediates ADP-induced intracellular calcium mobilization and shape change in platelets.

The Journal of Biological Chemistry
J JinS P Kunapuli

Abstract

ADP is an important platelet agonist causing shape change from smooth discoid shape to spiculated spheres and platelet aggregation. However, the molecular mechanisms involved in ADP-induced platelet activation have not been elucidated. We demonstrated earlier the existence of two distinct ADP receptors on platelets, one coupled to phospholipase C, P2TPLC, and the other to inhibition of adenylyl cyclase, P2TAC (Daniel, J. L., Dangelmaier, C., Jin, J., Ashby, B., Smith, J. B., and Kunapuli, S. P. (1998) J. Biol. Chem. 273, 2024-2029), in addition to the previously described P2X1 receptor. Here we report the cloning of a cDNA clone encoding the P2Y1 receptor from a human platelet cDNA library by homology screening with radiolabeled P2Y1-P2Y6 receptor cDNAs. ADP or 2-methyl(thio)-ADP-induced intracellular calcium increases were inhibited by the P2Y1 receptor-specific antagonists, adenosine 3'-phosphate 5'-phosphosulfate (A3P5PS), adenosine 3'-phosphate 5'-phosphate (A3P5P), and adenosine 2'-phosphate 5'-phosphate (A2P5P), in a concentration-dependent manner, but not by ARL 66096 or alpha, beta-MeATP. A3P5PS, A3P5P, and A2P5P also inhibited the shape change of aspirinated platelets induced by 10 microM ADP or 3 microM 2-methyl-(thio...Continue Reading

References

Jan 1, 1985·General Pharmacology·G Burnstock, C Kennedy
Jun 1, 1995·Trends in Pharmacological Sciences·R G HumphriesP Leff
May 24, 1995·Biochimica Et Biophysica Acta·N J GrecoG A Jamieson
Dec 29, 1995·The Journal of Biological Chemistry·T NguyenB F O'Dowd
Jan 26, 1996·Biochemical and Biophysical Research Communications·K AyyanathanS P Kunapuli
Feb 6, 1996·Biochemical and Biophysical Research Communications·T E WebbE A Barnard
Feb 9, 1996·The Journal of Biological Chemistry·A B MacKenzieS O Sage
Aug 2, 1996·The Journal of Biological Chemistry·G K AkbarS P Kunapuli
May 1, 1996·British Journal of Pharmacology·J B SchachterT K Harden
May 1, 1996·Journal of Receptor and Signal Transduction Research·G K AkbarS P Kunapuli
Sep 1, 1996·Somatic Cell and Molecular Genetics·K AyyanathanS P Kunapuli
Mar 1, 1997·Trends in Pharmacological Sciences·B B FredholmM Williams
Jul 1, 1997·Somatic Cell and Molecular Genetics·L V PidlaoanS P Kunapuli
Jan 1, 1996·Platelets·C GachetJ P Cazenave

❮ Previous
Next ❯

Citations

Apr 25, 2000·Biochemical and Biophysical Research Communications·I M FrancischettiC Bon
Jan 17, 2002·International Journal of Hematology·C Gachet
Sep 15, 2005·Naunyn-Schmiedeberg's Archives of Pharmacology·Susanne HornerGünter Lambrecht
Aug 19, 2011·Pflügers Archiv : European journal of physiology·Marianne SkalsHelle A Praetorius
Mar 28, 2008·Purinergic Signalling·David Erlinge, Geoffrey Burnstock
Apr 13, 2011·Purinergic Signalling·Kenneth A JacobsonStefano Costanzi
Apr 13, 2011·Purinergic Signalling·Martyn P Mahaut-SmithRichard J Evans
Apr 13, 2011·Purinergic Signalling·Marian A Packham, Margaret L Rand
Jul 28, 2011·Purinergic Signalling·Béatrice Hechler, Christian Gachet
Jan 12, 2013·Journal of Cardiovascular Translational Research·Giovanni Cimmino, Paolo Golino
May 24, 2005·Analytical Biochemistry·Louie TranDiana B Cherbavaz
Jun 16, 2005·Pharmacology & Therapeutics·Christian Gachet
Jun 1, 2005·Thrombosis Research·Sven NylanderTomas L Lindahl
Aug 1, 1998·European Journal of Pharmacology·J GeigerU Walter
Jul 8, 1999·European Journal of Pharmacology·S TakanoT Ono
Jun 28, 2000·Journal of the Autonomic Nervous System·B F King, A Townsend-Nicholson
Jun 28, 2000·Journal of the Autonomic Nervous System·N J Cusack, S M Hourani
Nov 6, 1998·Trends in Pharmacological Sciences·S P Kunapuli
Jan 22, 2004·Critical Reviews in Oncology/hematology·Indra Ramasamy
Oct 24, 1998·The International Journal of Biochemistry & Cell Biology·R N Puri
Apr 12, 2003·Current Opinion in Pharmacology·Satya P KunapuliTodd M Quinton
Nov 9, 2011·Nature Medicine·Shaun P Jackson
Feb 29, 2000·British Journal of Pharmacology·G E JarvisP Leff
Nov 14, 2002·British Journal of Pharmacology·Kerry L Homer, Janet C Wanstall
Oct 19, 2001·European Journal of Biochemistry·B T AtkinsonS P Watson
Apr 12, 2000·European Journal of Biochemistry·C DangelmaierS P Kunapuli
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·B HechlerK Ravid

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.