Molecular basis for sterol transport by StART-like lipid transfer domains

The EMBO Journal
Florian A HorenkampKarin M Reinisch

Abstract

Lipid transport proteins at membrane contact sites, where two organelles are closely apposed, play key roles in trafficking lipids between cellular compartments while distinct membrane compositions for each organelle are maintained. Understanding the mechanisms underlying non-vesicular lipid trafficking requires characterization of the lipid transporters residing at contact sites. Here, we show that the mammalian proteins in the lipid transfer proteins anchored at a membrane contact site (LAM) family, called GRAMD1a-c, transfer sterols with similar efficiency as the yeast orthologues, which have known roles in sterol transport. Moreover, we have determined the structure of a lipid transfer domain of the yeast LAM protein Ysp2p, both in its apo-bound and sterol-bound forms, at 2.0 Å resolution. It folds into a truncated version of the steroidogenic acute regulatory protein-related lipid transfer (StART) domain, resembling a lidded cup in overall shape. Ergosterol binds within the cup, with its 3-hydroxy group interacting with protein indirectly via a water network at the cup bottom. This ligand binding mode likely is conserved for the other LAM proteins and for StART domains transferring sterols.

References

May 10, 2000·Nature Structural Biology·Y Tsujishita, J H Hurley
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Michael J RomanowskiStephen K Burley
Aug 31, 2002·Biophysical Journal·Karin JohnAndreas Herrmann
Sep 27, 2002·Biophysical Journal·Theodore L SteckYvonne Lange
Jan 11, 2008·Proceedings of the National Academy of Sciences of the United States of America·Norio KudoRyuichi Kato
Jan 29, 2009·Proceedings of the National Academy of Sciences of the United States of America·Christer S EjsingAndrej Shevchenko
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Jul 9, 2011·PloS One·Ann-Gerd ThorsellLari Lehtiö
Sep 9, 2011·Molecular Biology of the Cell·Bruno MesminFrederick R Maxfield
Oct 4, 2011·The Journal of Endocrinology·Barbara J Clark
Dec 14, 2011·The Journal of Cell Biology·Maud de Saint-JeanGuillaume Drin
Oct 1, 2013·Biochimie·Fabien Alpy, Catherine Tomasetto
Dec 30, 2014·Molecular and Cellular Endocrinology·Danny LétourneauJean-Guy LeHoux
Jan 9, 2015·Current Opinion in Cell Biology·Sujoy LahiriWilliam A Prinz
Apr 8, 2015·Nature Communications·Joachim Moser von FilseckGuillaume Drin
May 20, 2015·The Journal of Cell Biology·Andrew MurleyJodi Nunnari
Jun 30, 2015·Cell Reports·Yael Elbaz-AlonMaya Schuldiner
Jul 15, 2015·Biochemistry·David B IaeaFrederick R Maxfield
Dec 22, 2015·Biochimica Et Biophysica Acta·Karin M Reinisch, Pietro De Camilli
Jan 27, 2016·International Review of Cell and Molecular Biology·Henriikka KentalaVesa M Olkkonen
Apr 12, 2016·Nature Cell Biology·Yasunori SahekiPietro De Camilli
Apr 14, 2016·Biochemical Society Transactions·Guillaume DrinAlenka Čopič
Aug 5, 2016·Acta Crystallographica. Section F, Structural Biology Communications·Martin P HorvathPaul S Bernstein
Jun 6, 2017·Trends in Biochemical Sciences·Louise H WongTim P Levine

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Citations

Jun 22, 2019·Nature Chemical Biology·Luca LaraiaHerbert Waldmann
Dec 5, 2019·ELife·George KhelashviliAnant K Menon
Apr 23, 2020·The Journal of Cell Biology·Magdalena MarekSophie G Martin
Apr 2, 2020·Biological Chemistry·Yasushi TamuraToshiya Endo
Jun 23, 2020·Annual Review of Biochemistry·Emma J FenechMaya Schuldiner
Oct 30, 2018·Journal of Biochemistry·Yasushi TamuraToshiya Endo
Apr 25, 2019·Journal of Lipid Research·Daniel Rodriguez-AgudoGregorio Gil
Feb 13, 2020·Frontiers in Microbiology·Svyatoslav S SokolovDmitry A Knorre
Sep 11, 2019·FEMS Microbiology Reviews·Alexandros AthanasopoulosChristos Gournas
Aug 16, 2019·Theoretical Biology & Medical Modelling·Daniel Wüstner
Oct 9, 2019·Annual Review of Cell and Developmental Biology·Tamas BallaJoshua Pemberton
Aug 15, 2020·Frontiers in Cell and Developmental Biology·Mohammad F ZamanChristopher T Beh
Oct 7, 2020·International Journal of Molecular Sciences·Sebastian Kreimendahl, Joachim Rassow
Jul 28, 2019·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Taki Nishimura, Christopher J Stefan
Oct 13, 2019·Atherosclerosis·Markus AxmannHerbert Stangl
Jan 14, 2020·Molecular and Cellular Endocrinology·Barbara J Clark
May 16, 2020·Trends in Cell Biology·Ying MengPaul Saftig
Mar 28, 2020·Current Opinion in Cell Biology·Joshua A Lees, Karin M Reinisch
Mar 9, 2021·Frontiers in Cell and Developmental Biology·Bailey HewlettThierry Galli
May 2, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Tomoki Naito, Yasunori Saheki
Jun 13, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Valentin SchoopDoris Höglinger
Aug 6, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Denisa Jamecna, Bruno Antonny
Apr 3, 2020·Journal of Chemical Information and Modeling·Laust MoesgaardJacob Kongsted

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