Abstract
A non-toxic protein (TsNTxP) isolated from the venom of the noxious scorpion Tityus serrulatus (Ts) induces polyclonal antibodies cross-reactive with several toxins from the venom, in sharp contrast to anti-toxin antibodies which are toxin specific. To try to uncover the molecular basis for these unusual properties, peptide scanning experiments were performed and indicated that the N- and C-terminal parts of TsNTxP enclose continuous epitopes (residues 1-15 and 47-61). Antibodies raised against peptides corresponding to these two regions were found to have neutralizing properties against a mixture of all toxic proteins from the T. serrulatus venom, indicating that residues 1-15 and 47-61 correspond to neutralizing epitopes. The identification of key antigenic residues within these two epitopes revealed that several of them are well conserved in the amino-acid sequences of the three main toxins (Ts II, Ts IV and Ts VII) from the venom: Glu 3, Tyr 5, Asp 8, Asp 50, Trp 55 and Lys 61. A single key-residue (Glu 58) is unique to TsNTxP. By using homology modeling, a model of the three-dimensional structure of TsNTxP was obtained. The antigenically important residues from TsNTxP were found to be surface exposed, with five of them clu...Continue Reading
References
Jan 1, 1992·Natural Toxins·P MansuelleC Granier
Jun 1, 1989·Molecular Immunology·C GranierE Bahraoui
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J C Fontecilla-CampsH Rochat
Dec 1, 1970·European Journal of Biochemistry·F MirandaS Lissitzky
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H M GeysenS J Barteling
Aug 16, 1984·Biochemical and Biophysical Research Communications·G BechisH Rochat
Dec 1, 1983·Biopolymers·W Kabsch, C Sander
Jan 1, 1982·Toxicon : Official Journal of the International Society on Toxinology·J C Fontecilla-CampsC E Bugg
Jan 1, 1981·Toxicon : Official Journal of the International Society on Toxinology·P DeloriH Rochat
Aug 29, 1980·Biochemical and Biophysical Research Communications·E JoverH Rochat
Jan 1, 1995·Revista Do Instituto De Medicina Tropical De São Paulo·N A de RezendeC F Amaral
Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell
Jul 1, 1993·International Journal of Peptide and Protein Research·J MéryJ Brugidou
Jul 1, 1993·Immunopharmacology·J M WitkowskiL Trawicka
Feb 1, 1993·Toxicon : Official Journal of the International Society on Toxinology·M E De LimaC Granier
Sep 1, 1995·Vaccine·E S Calderon-ArandaL D Possani
Jan 1, 1997·Journal of the American Society of Nephrology : JASN·Y Oh, D G Warnock
Dec 15, 1996·European Journal of Biochemistry·C DevauxC Granier
Feb 1, 1997·Toxicon : Official Journal of the International Society on Toxinology·C Chávez-OlórteguiC R Diniz
Jul 1, 1997·Proteins·C LandonM Ptak
Mar 21, 1998·Electrophoresis·N Guex, M C Peitsch
Jun 10, 1998·Toxicon : Official Journal of the International Society on Toxinology·A M Moreira-FerreiraC Chávez-Olórtegui
Mar 26, 1999·European Journal of Biochemistry·O HassaniF Sampieri
Jul 2, 1999·Journal of Molecular Biology·I PolikarpovA Teplyakov
Jun 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·Z F LaiK Nishi
Jul 21, 1962·Nature·R A REISFELDD E WILLIAMS
Citations
Oct 17, 2001·Toxicon : Official Journal of the International Society on Toxinology·L M AlvarengaC Chávez-Olórtegui
Nov 30, 2002·Toxicon : Official Journal of the International Society on Toxinology·Evanguedes KalapothakisCarlos Chávez-Olórtegui
Mar 20, 2012·Journal of Immunotoxicology·Manuela Berto PuccaJosé Elpidio Barbosa
Jul 15, 2015·Toxins·Manuela B PuccaEliane C Arantes
Mar 17, 2007·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·G D'SuzeA Alagón
Dec 2, 2009·Vaccine·Clara Guerra DuarteCarlos Chávez-Olórtegui
Sep 9, 2008·Toxicon : Official Journal of the International Society on Toxinology·T M MendesE Kalapothakis
Dec 7, 2007·Toxicon : Official Journal of the International Society on Toxinology·Najet Srairi-AbidMohamed El Ayeb
Oct 9, 2007·Toxicon : Official Journal of the International Society on Toxinology·Adolfo BorgesCarlos Chávez-Olórtegui
Apr 20, 2005·Vaccine·Karlen G GazarianLourival D Possani
Feb 3, 2005·Toxicon : Official Journal of the International Society on Toxinology·Elia Diego-GarcíaLourival D Possani
Apr 28, 2004·Toxicon : Official Journal of the International Society on Toxinology·Adolfo BorgesStefan H Heinemann
Dec 17, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Adolfo BorgesHuub J M Op den Camp
Dec 3, 2014·Toxicon : Official Journal of the International Society on Toxinology·K L CastroC Chávez-Olortegui
Jun 23, 2005·Toxicon : Official Journal of the International Society on Toxinology·W S MariaC Chávez-Olórtegui
Dec 8, 2009·Vaccine·L AlvarengaC Granier
Aug 30, 2011·Vaccine·J de MouraC Chávez-Olórtegui
Jul 19, 2011·Peptides·R A Machado de AvilaC Chávez-Olórtegui
Sep 20, 2005·Toxicon : Official Journal of the International Society on Toxinology·L M AlvarengaC Chávez-Olórtegui
Nov 24, 2017·Toxins·Daniela Cajado-CarvalhoFernanda V Portaro
Apr 19, 2018·Frontiers in Immunology·Sabrina de Almeida LimaCarlos Chávez-Olórtegui
May 31, 2017·PLoS Neglected Tropical Diseases·Thais Melo MendesCarlos Chávez-Olórtegui
Nov 8, 2018·Toxins·Erick Bermúdez-MéndezAndreas Hougaard Laustsen