Molecular basis for the cross-reactivity of antibodies elicited by a natural anatoxin with alpha- and beta-toxins from the venom of Tityus serrulatus scorpion

Molecular Immunology
Carlos Chávez-OlórteguiClaude Granier

Abstract

A non-toxic protein (TsNTxP) isolated from the venom of the noxious scorpion Tityus serrulatus (Ts) induces polyclonal antibodies cross-reactive with several toxins from the venom, in sharp contrast to anti-toxin antibodies which are toxin specific. To try to uncover the molecular basis for these unusual properties, peptide scanning experiments were performed and indicated that the N- and C-terminal parts of TsNTxP enclose continuous epitopes (residues 1-15 and 47-61). Antibodies raised against peptides corresponding to these two regions were found to have neutralizing properties against a mixture of all toxic proteins from the T. serrulatus venom, indicating that residues 1-15 and 47-61 correspond to neutralizing epitopes. The identification of key antigenic residues within these two epitopes revealed that several of them are well conserved in the amino-acid sequences of the three main toxins (Ts II, Ts IV and Ts VII) from the venom: Glu 3, Tyr 5, Asp 8, Asp 50, Trp 55 and Lys 61. A single key-residue (Glu 58) is unique to TsNTxP. By using homology modeling, a model of the three-dimensional structure of TsNTxP was obtained. The antigenically important residues from TsNTxP were found to be surface exposed, with five of them clu...Continue Reading

References

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Citations

Oct 17, 2001·Toxicon : Official Journal of the International Society on Toxinology·L M AlvarengaC Chávez-Olórtegui
Nov 30, 2002·Toxicon : Official Journal of the International Society on Toxinology·Evanguedes KalapothakisCarlos Chávez-Olórtegui
Mar 20, 2012·Journal of Immunotoxicology·Manuela Berto PuccaJosé Elpidio Barbosa
Mar 17, 2007·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·G D'SuzeA Alagón
Sep 9, 2008·Toxicon : Official Journal of the International Society on Toxinology·T M MendesE Kalapothakis
Dec 7, 2007·Toxicon : Official Journal of the International Society on Toxinology·Najet Srairi-AbidMohamed El Ayeb
Oct 9, 2007·Toxicon : Official Journal of the International Society on Toxinology·Adolfo BorgesCarlos Chávez-Olórtegui
Apr 20, 2005·Vaccine·Karlen G GazarianLourival D Possani
Feb 3, 2005·Toxicon : Official Journal of the International Society on Toxinology·Elia Diego-GarcíaLourival D Possani
Apr 28, 2004·Toxicon : Official Journal of the International Society on Toxinology·Adolfo BorgesStefan H Heinemann
Dec 17, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Adolfo BorgesHuub J M Op den Camp
Dec 3, 2014·Toxicon : Official Journal of the International Society on Toxinology·K L CastroC Chávez-Olortegui
Jun 23, 2005·Toxicon : Official Journal of the International Society on Toxinology·W S MariaC Chávez-Olórtegui
Sep 20, 2005·Toxicon : Official Journal of the International Society on Toxinology·L M AlvarengaC Chávez-Olórtegui
Nov 8, 2018·Toxins·Erick Bermúdez-MéndezAndreas Hougaard Laustsen

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