Abstract
Thrombin is a mammalian serine proteinase that plays a prominent role in the maintenance and regulation of hemostasis through its interaction with various substrates and/or ligands. The venoms of several snakes contain glycosylated serine proteinases that have been recognized to possess one or more of the essential activities of thrombin on fibrinogen (Fg) and/or platelets. These proteinases share about 60% sequence identity. One class of snake venom serine proteinases are those known as thrombin-like (TLE), named after their ability to directly clot Fg in order to preferentially produce fibrinopeptide A, fibrinopeptide B or both. To understand the molecular basis of this phenomenon, the corresponding amino acid sequences and molecular structures need to be analyzed. Given the absence of experimentally determined tertiary structures of snake venom, TLEs, three-dimensional molecular models should prove useful in this context. Towards this goal, we obtained models of snake venom TLEs that used TSV-PA as template, TSV-PA being the only snake venom serine proteinase whose crystal structure is known to date. Along with a comparative sequence analysis the models contribute to the identification and description of thrombin-homologous ...Continue Reading
Citations
Dec 23, 2003·Journal of Molecular Biology·Peter HägglöfTor Ny
Jan 9, 2004·Protein Expression and Purification·Göran Karlsson, Stefan Winge
Jun 24, 2003·Thrombosis Research·Benilde CosmiSergio Coccheri
Oct 3, 2002·Protein Expression and Purification·Göran Karlsson, Stefan Winge
Mar 26, 2003·Protein Expression and Purification·Göran Karlsson, Stefan Winge
Sep 10, 2003·Protein Expression and Purification·Hak-Joo Lee, Hana Im
Oct 2, 2003·European Journal of Biochemistry·George L LongJohan Stenflo
Dec 4, 2004·Protein Science : a Publication of the Protein Society·Yu-Ran Na, Hana Im
Jan 1, 2010·Thrombosis·Isis S R CarterRoss T A Macgillivray
Dec 3, 2014·Journal of Proteomics·André ZelanisChristopher M Overall
Feb 3, 2005·Toxicon : Official Journal of the International Society on Toxinology·Yang JinYu-Liang Xiong
Oct 13, 2006·Biochimica Et Biophysica Acta·Eladio F SanchezMichael Richardson
Feb 21, 2006·Toxicon : Official Journal of the International Society on Toxinology·Henrique P B MagalhãesEdyr Rogana
Feb 17, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Arinos MagalhãesEladio F Sanchez
Jun 1, 2005·Toxicon : Official Journal of the International Society on Toxinology·Solange M T Serrano, Rachid C Maroun
Nov 25, 2003·The American Journal of Pathology·Gwenaële GarinJohn L McGregor
Dec 7, 2005·Acta Biochimica Et Biophysica Sinica·Hao-Mang JiaoYuan-Cong Zhou