Molecular basis of growth hormone daily mRNA and protein synthesis in rats

Life Sciences
T de Castro BarbosaMaria Tereza Nunes

Abstract

Daily and seasonal rhythms coordinate the endocrine and metabolic functions. The pituitary gland is the master regulator of several endocrine activities, and its function is classically regulated by endocrine signals from its target glands as well as from the hypothalamus. The growth hormone (GH) produced and secreted by the anterior pituitary presents a pulsatile secretion throughout the 24-hour cycle. However, the molecular mechanisms regulating the daily pattern of GH secretion are still unclear. Herein we investigated whether circadian GH mRNA and protein synthesis is modulated by acute adjustments in the stability and expression of GH mRNA. GH mRNA and protein content were evaluated by real-time PCR and Western blotting, respectively, in pituitary gland of rats euthanized every 3 h during a 24-h period at the Zeitgeber times (ZT3 to ZT24). The GH mRNA poly(A) tail length was determined by RACE-PAT assay. We identified two main peaks of GH mRNA level in the pituitary gland of rats; one in the middle of the light-cycle and another in the middle of the dark-cycle. The latter was associated with an increase in pituitary GH protein content. Interestingly, an increment in the poly(A) tail length of the GH transcript was observed...Continue Reading

References

Oct 1, 1979·Journal of Endocrinological Investigation·G TolisH Guyda
Aug 1, 1975·The American Journal of Physiology·B H NatelsonP E Stokes
Jun 1, 1990·The Journal of Clinical Endocrinology and Metabolism·L M WinerG Baumann
Jul 1, 1987·Physiological Reviews·E E Müller
Sep 1, 1986·Metabolism: Clinical and Experimental·M W Stolar, G Baumann
Jun 1, 1969·The Journal of Clinical Endocrinology and Metabolism·D C ParkerL G Rossman
Sep 1, 1968·The Journal of Clinical Investigation·Y TakahashiW H Daughaday
Jan 1, 1996·Domestic Animal Endocrinology·C K Tuggle, A Trenkle
Apr 1, 1997·Current Opinion in Genetics & Development·M WickensR J Jackson
Mar 17, 1999·Methods : a Companion to Methods in Enzymology·F J SallésS Strickland
Mar 23, 2002·Cell·Nick J ProudfootMichael J Dye
Oct 16, 2004·Annual Review of Genomics and Human Genetics·Phillip L Lowrey, Joseph S Takahashi
Mar 13, 2008·Nature Reviews. Molecular Cell Biology·Aaron C Goldstrohm, Marvin Wickens
Sep 9, 2008·Cell·Carla B GreenJoseph Bass
May 19, 2010·Progress in Brain Research·Earn-Hui Gan, Richard Quinton
May 23, 2012·Trends in Endocrinology and Metabolism : TEM·Jeremy J StubblefieldCarla B Green
Dec 20, 2012·Genes & Development·Shihoko KojimaCarla B Green
Jun 19, 2013·Nature Reviews. Genetics·Ran ElkonReuven Agami
Oct 5, 2013·Trends in Genetics : TIG·Bijoyita Roy, Allan Jacobson
May 28, 2014·Nature Reviews. Endocrinology·Karen L GambleMartin E Young
Oct 15, 2014·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Rafael Barrera SalgueiroMaria Tereza Nunes
Feb 11, 2015·Molecular and Cellular Endocrinology·Pawan Kumar JhaAndries Kalsbeek
Apr 24, 2016·The Journal of Endocrinology·Anthony H TsangHenrik Oster

❮ Previous
Next ❯

Citations

Feb 13, 2019·Thyroid : Official Journal of the American Thyroid Association·Paula Bargi-SouzaMaria Tereza Nunes
Aug 27, 2021·Metabolites·Blandine TramuntWalter Wahli

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Aphasia

Aphasia affects the ability to process language, including formulation and comprehension of language and speech, as well as the ability to read or write. Here is the latest research on aphasia.