Molecular basis of halorespiration control by CprK, a CRP-FNR type transcriptional regulator.

Molecular Microbiology
Colin LevyDavid Leys

Abstract

Certain bacteria are able to conserve energy via the reductive dehalogenation of halo-organic compounds in a respiration-type metabolism. The transcriptional regulator CprK from Desulfitobacterium spp. induces expression of halorespiratory genes upon binding of o-chlorophenol ligands and is reversibly inactivated by oxygen through disulphide bond formation. We report crystal structures of D. hafniense CprK in the ligand-free (both oxidation states), ligand-bound (reduced) and DNA-bound states, making it the first member of the widespread CRP-FNR superfamily for which a complete structural description of both redox-dependent and allosteric molecular rearrangements is available. In conjunction with kinetic and thermodynamic ligand binding studies, we provide a model for the allosteric mechanisms underpinning transcriptional control. Amino acids that play a key role in this mechanism are not conserved in functionally distinct CRP-FNR members. This suggests that, despite significant structural homology, distinct allosteric mechanisms are used, enabling this protein family to control a very wide range of processes.

References

Dec 20, 1993·Journal of Molecular Biology·M C Lawrence, P M Colman
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·W K OlsonV B Zhurkin
Oct 4, 2000·Nature Structural Biology·W N LanzilottaT L Poulos
May 10, 2001·Biochimica Et Biophysica Acta·J G Harman
Jun 16, 2001·Advances in Microbial Physiology·J GreenJ R Guest
Oct 17, 2001·Journal of Molecular Biology·W K OlsonH M Berman
Aug 31, 2002·Science·Brian BenoffRichard H Ebright
Oct 7, 2003·Annual Review of Biochemistry·Ruma Banerjee, Stephen W Ragsdale
Apr 23, 2004·Current Opinion in Structural Biology·Catherine L LawsonRichard H Ebright
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Sep 24, 2004·The Journal of Biological Chemistry·Stelian M PopStephen W Ragsdale
Oct 19, 2004·Annual Review of Microbiology·Hauke Smidt, Willem M de Vos
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Jan 8, 2005·Science·Rekha SeshadriJohn F Heidelberg
Jun 29, 2006·The Journal of Biological Chemistry·M Gordon JoyceDavid Leys
Aug 15, 2006·Nature Structural & Molecular Biology·Nataliya PopovychCharalampos G Kalodimos
Aug 17, 2006·FEMS Microbiology Reviews·Richard VillemurFrançois Lépine
Feb 17, 2007·The Journal of Biological Chemistry·Hortense MazonRobert H H van den Heuvel
Mar 1, 2007·Acta Crystallographica. Section D, Biological Crystallography·Mandula BorjiginThomas L Poulos

❮ Previous
Next ❯

Citations

Apr 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Nataliya PopovychCharalampos G Kalodimos
Dec 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Socorro MesaHauke Hennecke
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hitesh SharmaThomas A Steitz
Jul 16, 2010·Antioxidants & Redox Signaling·Haike Antelmann, John D Helmann
Mar 20, 2012·Antioxidants & Redox Signaling·Serena RinaldoFrancesca Cutruzzolà
Jul 19, 2011·Nucleic Acids Research·Ricardo AramayoElena V Orlova
Mar 13, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Anke WagnerUte Lechner
Mar 13, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Laura R KempDavid Leys
Mar 13, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Aamani RupakulaJulien Maillard
Jul 11, 2014·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Francesca CutruzzolàSerena Rinaldo
Aug 27, 2014·Journal of Molecular Biology·João H Morais-Cabral, Gail A Robertson
Dec 3, 2014·Journal of Molecular Biology·Stephen S MacKinnon, Shoshana J Wodak
Mar 19, 2016·Cell Chemical Biology·James A D GoodJörgen Johansson
Sep 28, 2015·Trends in Biotechnology·Bat-Erdene JugderChristopher P Marquis
Mar 16, 2013·Current Opinion in Biotechnology·Ruth Ellen Richardson
Jul 19, 2013·The Journal of Biological Chemistry·Claudia M Cremers, Ursula Jakob
Mar 7, 2014·Acta Crystallographica. Section D, Biological Crystallography·Truc KimKyeong Kyu Kim
Sep 6, 2019·Microbiology and Molecular Biology Reviews : MMBR·Ahmed GaballaKathryn J Boor
Apr 3, 2013·The Journal of Biological Chemistry·Mariette BonnetMarkus G Grütter
Dec 9, 2016·Proceedings of the National Academy of Sciences of the United States of America·Michael HallA Elisabeth Sauer-Eriksson
Mar 21, 2020·Frontiers in Microbiology·Mathilde Stéphanie WilleminJulien Maillard
Apr 17, 2019·Acta Crystallographica. Section D, Structural Biology·B Ross BelvinJanina P Lewis
Jul 14, 2017·Frontiers in Cellular and Infection Microbiology·Changyong ChengHouhui Song
Jun 25, 2015·Microbiology Spectrum·Esteban Martínez-GarcíaVíctor De Lorenzo

❮ Previous
Next ❯

Methods Mentioned

BETA
isothermal titration calorimetry

Software Mentioned

Phaser
Refmac
COOT
Spectrakinetics
XDS
CCP4

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.