Molecular basis of the 14-3-3 protein-dependent activation of yeast neutral trehalase Nth1

Proceedings of the National Academy of Sciences of the United States of America
Miroslava AlblovaT Obsil

Abstract

The 14-3-3 proteins, a family of highly conserved scaffolding proteins ubiquitously expressed in all eukaryotic cells, interact with and regulate the function of several hundreds of partner proteins. Yeast neutral trehalases (Nth), enzymes responsible for the hydrolysis of trehalose to glucose, compared with trehalases from other organisms, possess distinct structure and regulation involving phosphorylation at multiple sites followed by binding to the 14-3-3 protein. Here we report the crystal structures of yeast Nth1 and its complex with Bmh1 (yeast 14-3-3 isoform), which, together with mutational and fluorescence studies, indicate that the binding of Nth1 by 14-3-3 triggers Nth1's activity by enabling the proper 3D configuration of Nth1's catalytic and calcium-binding domains relative to each other, thus stabilizing the flexible part of the active site required for catalysis. The presented structure of the Bmh1:Nth1 complex highlights the ability of 14-3-3 to modulate the structure of a multidomain binding partner and to function as an allosteric effector. Furthermore, comparison of the Bmh1:Nth1 complex structure with those of 14-3-3:serotonin N-acetyltransferase and 14-3-3:heat shock protein beta-6 complexes revealed simila...Continue Reading

References

Oct 1, 1996·Journal of Molecular Evolution·W Wang, D C Shakes
Oct 23, 1997·The Journal of Biological Chemistry·U BanikS Kaufman
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·L ZhangH Fu
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·S GangulyD C Klein
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Jan 26, 2005·Biochemical Society Transactions·N AvonceG Iturriaga
Oct 6, 2005·IUBMB Life·G Paul H van Heusden
Nov 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Xiaowen YangJonathan M Elkins
May 7, 2009·The Journal of Biological Chemistry·Jan SilhanTomas Obsil
Jan 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Vincent B ChenDavid C Richardson
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Feb 10, 2010·The Biochemical Journal·Catherine JohnsonCarol MacKintosh
Apr 24, 2010·FEMS Microbiology Reviews·Wayne A WilsonJavier Pozueta-Romero
Aug 2, 2011·Nature·Ken-ichiro TaokaKo Shimamoto
Sep 17, 2011·Seminars in Cell & Developmental Biology·Tomas Obsil, Veronika Obsilova
Jun 4, 2013·Biochimica Et Biophysica Acta·Eva MacakovaVeronika Obsilova
Apr 10, 2014·The Journal of Biological Chemistry·Miroslava KopeckaVeronika Obsilova

❮ Previous
Next ❯

Citations

Oct 4, 2018·The FEBS Journal·Aneta SmidovaVeronika Obsilova
Jul 28, 2018·Journal of Biomolecular Structure & Dynamics·Meenakshi B TellisRakesh S Joshi
Jan 22, 2020·The FEBS Journal·Dana KalabovaVeronika Obsilova
Jan 12, 2020·Applied Microbiology and Biotechnology·Masayoshi Sakaguchi
Feb 28, 2020·Molecular & Cellular Proteomics : MCP·Michael PlankRobbie Loewith
Aug 28, 2020·The FEBS Journal·Veronika Obsilova, Tomas Obsil
Jan 11, 2019·Physiological Research·M AlblovaV Obsilova
Oct 20, 2019·Scientific Reports·Kristina V TugaevaNikolai N Sluchanko
Dec 10, 2019·MedChemComm·Dario ValentiChristian Ottmann
Sep 19, 2018·Nature Communications·Tobias KarlbergHerwig Schüler
Jul 22, 2019·International Journal of Molecular Sciences·Xiaolan FanMingyao Yang
Nov 26, 2020·International Journal of Molecular Sciences·Veronika Obsilova, Tomas Obsil
Jan 15, 2021·Scientific Reports·Lisa DenglerJennifer C Ewald
Jan 17, 2021·Molecular & Cellular Proteomics : MCP·Michael PlankRobbie Loewith
Jul 24, 2021·Communications Biology·Pavel PohlVeronika Obsilova
Jul 25, 2021·Journal of Molecular Biology·Seppe LeysenJeremy Martin Davis
Mar 24, 2020·ACS Omega·Madita WolterChristian Ottmann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.