Molecular basis of the isozymes of bovine glucose-6-phosphate isomerase

Archives of Biochemistry and Biophysics
J K Cini, R W Gracy

Abstract

Glucose-6-phosphate isomerase occurs in different bovine tissues as multiple, catalytically active isozymes which can be resolved by polyacrylamide gel electrophoresis (PAGE) and isoelectric focusing (IEF). Most differentiated tissues have five distinct forms with apparent pI values of 7.2, 7.0, 6.8, 6.6, and 6.4. Young, mitotically active, cells of the intestinal mucosa and the epithelium of the eye lens show only the two more basic isozymes, while old cells in the cortex and nucleus of the eye lens accumulate the more acidic isozymes. All of the isozymes exhibit equal separation based on charge-to-mass ratio (PAGE) and charge (IEF), thus indicating only charge changes. The isozyme patterns are unchanged in the presence of reducing agents or protease inhibitors. Each isozyme was purified to homogeneity and shown to exhibit identical subunit molecular weights (59,000) on SDS-gel electrophoresis. Each of the isolated isozymes, when subjected to PAGE or IEF, exhibited a single band, indicating that the isozymes are not generated as a result of electrophoresis. When the most basic isozyme was incubated in vitro under mild alkaline conditions, there was a spontaneous generation of the more acidic isozymes with properties identical ...Continue Reading

References

Oct 1, 1977·Archives of Biochemistry and Biophysics·M V KesterR W Gracy
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Citations

Dec 1, 1988·Molecular & General Genetics : MGG·J B GreenB S Hartley
Jun 15, 1989·Clinica Chimica Acta; International Journal of Clinical Chemistry·M F MorrisR L Van Etten
Jan 1, 1995·Molecular Aspects of Medicine·S I Rattan
Oct 19, 1989·Biochimica Et Biophysica Acta·A FaziM Magnani
Jan 1, 1996·Experimental Gerontology·S I Rattan
Nov 21, 1992·Annals of the New York Academy of Sciences·S I RattanB F Clark
Jul 1, 1997·Journal of the American Geriatrics Society·A Gafni
May 15, 1988·Archives of Biochemistry and Biophysics·J K CiniR W Gracy

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