Molecular biological detection and characterization of Clostridium populations in municipal landfill sites.

Applied and Environmental Microbiology
Michele I Van Dyke, Alan J McCarthy

Abstract

Primer sets specific for 16S rRNA genes were designed for four phylogenetic groups of clostridia known to contain mesophilic cellulolytic species. Specific amplification of these groups from landfill leachate DNA extracts demonstrated the widespread occurrence of clostridia from the Clostridium thermocellum and C. leptum groups. In contrast, the C. botulinum group was never detected, and the C. coccoides-C. lentocellum group was only occasionally detected. Amplification products were analyzed by temporal thermal gel electrophoresis to generate profiles of the clostridial groups and to identify dominant bands. Sequence analysis of 17 landfill clones confirmed that the primers were specific for the clostridial subgroups and that the cloned sequences had a close relationship with known cellulose-degrading clostridia. The primers have therefore been authenticated for use in the rapid identification of clostridia in anaerobic environments.

References

Jan 1, 1990·Methods in Enzymology·W R Pearson
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D J LaneN R Pace
Jun 1, 1987·Microbiological Reviews·C R Woese
Sep 1, 1994·Computer Applications in the Biosciences : CABIOS·Y Van de Peer, R De Wachter
Oct 1, 1994·International Journal of Systematic Bacteriology·M D CollinsJ A Farrow
Jan 1, 1995·Annual Review of Microbiology·S B Leschine
Apr 1, 1997·Applied and Environmental Microbiology·N K FryD A Stahl
Apr 1, 1997·International Journal of Systematic Bacteriology·F A RaineyM S da Costa
Dec 10, 1998·Nucleic Acids Research·B L MaidakC R Woese
May 20, 1999·Molecular Biology and Evolution·R DuncanN W Cornell
Dec 11, 1999·Nucleic Acids Research·D A BensonD L Wheeler

❮ Previous
Next ❯

Citations

Jan 4, 2012·Microbial Ecology·Emma Ransom-JonesJames E McDonald
Dec 7, 2010·Applied Microbiology and Biotechnology·Jeremy D Semrau
Oct 19, 2011·Proceedings of the National Academy of Sciences of the United States of America·Lifeng ZhuFuwen Wei
Nov 26, 2010·The Journal of Experimental Medicine·Andrea FuhrerThierry Hennet
Jun 9, 2009·Applied and Environmental Microbiology·James E McDonaldAlan J McCarthy
Sep 2, 2008·Applied and Environmental Microbiology·Marie-Claude JulienPascal Drouin
Aug 4, 2006·Applied and Environmental Microbiology·Robert J LockhartAlan J McCarthy
Nov 6, 2004·Applied and Environmental Microbiology·Yuli SongSydney M Finegold
May 5, 2006·Applied and Environmental Microbiology·Pascal PeuPatrick Dabert
Jun 8, 2012·BMC Microbiology·Marco CandelaPatrizia Brigidi
Nov 26, 2008·The Journal of General and Applied Microbiology·Natalie VaismanLucy Seldin
Aug 9, 2013·Indian Journal of Pathology & Microbiology·J Timko
Nov 1, 2013·European Journal of Nutrition·Bojana Bogovič MatijašićIrena Rogelj
Dec 4, 2012·Enzyme and Microbial Technology·I IeropoulosJ Greenman
May 23, 2012·Journal of Bioscience and Bioengineering·Nguyen Nhu SangMichihiko Ike
Mar 19, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Lisha ZhouHui Xu
Nov 26, 2015·International Journal of Systematic and Evolutionary Microbiology·Qi YinDaping Li
Nov 17, 2007·Bioresource Technology·C O'SullivanL L Blackall
May 8, 2007·Chemosphere·Lorraine MuckianNicholas Clipson
Aug 24, 2007·Journal of Applied Microbiology·D G CirneL L Blackall
Sep 28, 2007·Journal of Applied Microbiology·Y GuoC Deng
Jul 1, 2012·Asian-Australasian Journal of Animal Sciences·Yan GuoNengwu Zhu
May 20, 2015·Applied Microbiology and Biotechnology·Liyan SongYu Lei
Apr 5, 2014·TheScientificWorldJournal·Fayyaz Ali ShahSaeed Ahmad Asad
Nov 1, 2004·FEMS Microbiology Ecology·Li-Nan HuangLiang-Hu Qu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Botulism (ASM)

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.

Botulism

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.