PMID: 8984177Jan 1, 1996

Molecular biology approaches to evaluate species variation in immunogenicity and antigenicity of zona pellucida proteins

Journal of Reproduction and Fertility. Supplement
S V PrasadB S Dunbar

Abstract

Immunocontraception using the glycoproteins of the mammalian zona pellucida (ZP) has held great promise because antibodies specific to ZP antigens would inhibit fertility and not be abortive. It has been shown, however, that some ZP proteins will elicit adverse effects since immunization may affect ovarian follicular development. These effects vary among different mammalian species as well as on the source of the ZP immunogen. Therefore, the use of molecular biology has been essential in identifying specific ZP protein(s) that inhibit fertility without altering ovarian follicular development and in defining the relationships of ZP proteins among different species. Use of recombinant ZP proteins has allowed us to begin to dissect antigenic domains of ZP proteins and to evaluate their potential roles in the fertilization process. Recent studies using recombinant rabbit ZP proteins to immunize cynomolgus monkeys (Macaca fascicularis) have shown that the 55 kDa ZP protein will elicit antibodies that inhibit sperm binding while not altering ovarian function, in contrast to immunization with a recombinant truncated protein of the 75 kDa ZP protein which causes ovarian dysgenesis. The rabbit 55 kDa protein is the homologue of the pig ...Continue Reading

Related Concepts

Immunocontraceptive Techniques
Egg White Proteins
Macaca fascicularis
Membrane Glycoproteins
Hormone Receptors, Cell Surface
Proteins, Recombinant DNA
Zona Pellucida
Epitope Mapping

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