Abstract
The electrophoretic patterns of hexokinase and phosphoglucomutase have been widely used to distinguish Entamoeba histolytica from Entamoeba dispar isolates. Although E. histolytica and E. dispar, previously called pathogenic and nonpathogenic Entamoeba histolytica, differ clearly in sequences of many homologous genes, a conversion between the two has been reported by several laboratories, in each case showing the conversion of hexokinase (ATP, D-hexose 6-phosphotransferase, EC 2.7.1.1) isoenzyme patterns. An apparent mobility shift of this enzyme may either be due to posttranslational modification or processing, or to the appearance of a new isoform encoded by a second gene. In this study we observed that the four observed bands in the isoenzyme patterns of pathogenic and nonpathogenic forms of Entamoeba were correlated with four different cDNAs, and that the four recombinant hexokinases produced in Escherichia coli comigrated with their natural counterparts. Polymerase chain reaction (PCR) experiments did not reveal hidden genes which might be responsible for conversion phenomena. These results strongly support the redefinition of pathogenic and nonpathogenic Entamoeba histolytica as two closely related species Entamoeba histo...Continue Reading
Citations
Nov 30, 2006·Wiener klinische Wochenschrift·David LeitschMichael Duchêne
Aug 21, 2012·Indian Journal of Gastroenterology : Official Journal of the Indian Society of Gastroenterology·Gourdas Choudhuri, Murali Rangan
Mar 25, 2000·International Journal for Parasitology·A BhattacharyaS Bhattacharya
Mar 13, 1998·Molecular and Biochemical Parasitology·S OrtnerM Duchêne
May 26, 1999·Molecular and Biochemical Parasitology·R SharmaA Bhattacharya
Dec 29, 1999·Molecular and Biochemical Parasitology·H KroschewskiM Duchêne
Mar 16, 2001·Gene·G WuM Müller
Nov 18, 2011·Parasite : Journal De La Société Française De Parasitologie·J SuzukiT Takeuchi
Jan 9, 2001·Clinical Microbiology Reviews·P Upcroft, J A Upcroft
May 16, 2002·Infection and Immunity·Katrina Van DellenJohn Samuelson
Jan 12, 2008·Bioinformation·Shailza SinghDurlabh Kumar Sharma
Jan 13, 2000·Experimental Parasitology·H Tachibana, X J Cheng
Sep 19, 2006·Parasitology International·Elham RazmjouTomoyoshi Nozaki
Mar 29, 2013·Journal of Enzyme Inhibition and Medicinal Chemistry·María Leticia Saucedo-MendiolaAlfredo Téllez-Valencia
Oct 8, 2013·PloS One·Lewis R DartnellJan-Peter Muller
Apr 3, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·Mark BlaxterUNKNOWN Filarial Genome Project
Jul 23, 1998·Revista Do Instituto De Medicina Tropical De São Paulo·C M MottaL B Carvalho Júnior
Oct 4, 2000·Journal of Clinical Microbiology·S GhoshJ Samuelson
Jul 28, 2009·Experimental Parasitology·Jean-François Rossignol