Molecular biology of the human pyruvate dehydrogenase complex: structural aspects of the E2 and E3 components.

Annals of the New York Academy of Sciences
T J ThekkumkaraM S Patel

Abstract

The availability of the primary amino acid sequences of the E2 of PDC, alpha-KGDC and BCKADC from several prokaryotic and eukaryotic species has allowed us to compare the structural aspects of human PDC-E2 with those of the E2 components from the other complexes. The PDC-E2 components from all the species examined so far contain three structurally identifiable regions: the lipoyl-bearing domain, the E3-binding site, and the catalytic domain. The primary structure of the lipoyl-bearing domain shows considerable variation in its size, ranging from one to three repeating units of approximately 110 amino acids, but essentially preserving its function in the E2 components. In contrast, the sizes of the E3-binding site and the catalytic domain of PDC-E2 from several species are essentially similar and show considerable conservation of specific amino acid residues. Obviously, additional studies are warranted to better understand the structure-function relationships of these domains and the evolutionary conservation of PDC-E2 in different species. Similarly, the availability of the primary amino acid sequences of E3 from several prokaryotes and eukaryotes has also permitted comparison of the structural domains of these proteins with th...Continue Reading

References

Jun 15, 1987·Biochemical and Biophysical Research Communications·T J ThekkumkaraM S Patel
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·X D NiuL J Reed
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R L CoppelM E Gershwin
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G PonsM S Patel
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K S BrowningL J Reed
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A G LeslieW V Shaw
Jan 1, 1983·Reviews of Physiology, Biochemistry and Pharmacology·O H Wieland
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Apr 15, 1984·Journal of Molecular Biology·D W RiceJ R Guest
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Jan 1, 1982·European Journal of Biochemistry·R L Krauth-SiegelR Untucht-Grau
Nov 15, 1981·Journal of Molecular Biology·R ThiemeG E Schulz

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Citations

Sep 1, 1992·Proteins·J E JentoftM S Patel
Oct 21, 1994·Biochimica Et Biophysica Acta·I D WexlerD S Kerr
May 1, 1995·The International Journal of Biochemistry & Cell Biology·T Pawelczyk, M S Olson
Oct 27, 2010·Immunology and Cell Biology·Carlo SelmiM Eric Gershwin

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