Molecular biology of vertebrate transcription factor IIIA: cloning and characterization of TFIIIA from channel catfish oocytes

Gene
M K Ogilvie, J S Hanas

Abstract

TFIIIA regulates 5S rRNA synthesis and is the prototype of the Cys2His2 superfamily of zinc finger proteins. Because the TFIIIA aa sequence is highly diverged, elucidating species variation in this factor will yield insights into how zinc fingers bind DNA and how this protein regulates RNAPIII transcription. This study reports the identification, cloning and functional divergence of oocyte TFIIIA from the channel catfish. Catfish oocyte TFIIIA was identified by its association with 5S rRNA in immature ovarian tissue, its molecular weight, and by peptide sequence similarities with Xenopus TFIIIA. The cDNA for this factor was cloned by degenerate PCR and found to code for nine Cys2His2 zinc fingers and a C-terminal tail; only about 40% aa sequence identity was observed with Xenopus TFIIIA. The N-terminal region of catfish TFIIIA contains the oocyte-specific initiating Met amino acid and accompanying conserved residues found in amphibian TFIIIAs but not found in yeast or human TFIIIAs. Catfish TFIIIA lacks the conserved transcription activation domain in its C-terminal tail found in amphibian and human TFIIIA. Catfish TFIIIA was able to bind the catfish and Xenopus 5S RNA genes but did not efficiently promote 5S gene transcription...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Sep 20, 1992·Journal of Molecular Biology·J J Hayes, T D Tullius
Nov 13, 1992·Cell·O TheunissenT Pieler
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K R ClemensJ M Gottesfeld
May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·N A Woychik, R A Young
Apr 25, 1990·Nucleic Acids Research·C J Gaskins, J S Hanas
Jan 1, 1990·Annual Review of Biophysics and Biophysical Chemistry·J M Berg
Nov 26, 1990·European Journal of Biochemistry·R WaldschmidtK H Seifart
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A FrohmanG R Martin
Dec 11, 1989·Nucleic Acids Research·J S HanasR Zebrowski
Apr 1, 1988·Molecular and Cellular Biology·K E VranaD D Brown
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J S HanasC W Wu
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·H R Pelham, D D Brown
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y Choo, A Klug
Dec 1, 1993·Molecular and Cellular Biology·X Mao, M K Darby
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J R Desjarlais, J M Berg
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·R A FridellB R Cullen

❮ Previous
Next ❯

Citations

Jul 31, 2003·Journal of Molecular Biology·Deborah B Schulman, David R Setzer
Jul 10, 1998·Biochimica Et Biophysica Acta·J S HanasJ Q Wickham
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·W Zhang, J J Bieker
Apr 11, 2000·Nucleic Acids Research·R J MorelandJ S Hanas
Apr 25, 2002·Nucleic Acids Research·Nicholas Polakowski, Marvin R Paule
Jun 28, 2002·Nucleic Acids Research·Deborah B Schulman, David R Setzer
Nov 13, 2012·Biochimica Et Biophysica Acta·Elodie LayatSylvette Tourmente
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·R T NolteR S Brown
Jul 10, 2003·The Plant Journal : for Cell and Molecular Biology·Catherine CloixSylvette Tourmente
May 30, 2001·Biochemical Pharmacology·J S RodgersJ S Hanas
Oct 26, 1999·Molecular Pharmacology·J S HanasY G Cheng

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.