Molecular changes to the rat renal cotransporters PEPT1 and PEPT2 due to ageing

Molecular and Cellular Biochemistry
Othman A AlghamdiPierre D J Moens

Abstract

Renal PEPT1 and PEPT2 cotransporters play an important role in the balance of circulating body oligopeptides and selected peptidomimetic drugs. We aim to comprehensively characterise age-related changes of the renal PEPT cotransporters at the gene, protein, and functional level. Brush border membrane vesicles (BBMV) and outer medulla membrane vesicles (OMMV) were isolated from the kidneys of young, middle-aged and old rats. The protein expression of PEPT1 was not only increased in BBMV from old rats, but PEPT1 also appeared in OMMV from middle-aged and old rats. SLC15A1 gene expression in the renal cortex increased in middle-aged group. PEPT2 protein expression was not only increased with ageing, but PEPT2 also was found in BBMV from middle-aged and old groups. SLC15A2 gene expression in the renal outer medulla increased in the old group. These changes in the expressions and localisations of PEPT1 and PEPT2 could explain the changes to transport activity in BBMV and OMMV. These findings provide novel insights that would be useful for maintaining protein nutrition and optimising the delivery of some peptidomimetic drugs in elderly individuals.

References

Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·C J Seal, D S Parker
Dec 15, 1989·The Biochemical Journal·O I AruomaB Halliwell
Dec 1, 1985·Mechanisms of Ageing and Development·M NakanoH Tauchi
Jun 1, 1993·Pflügers Archiv : European journal of physiology·C D BrownN L Simmons
Aug 26, 1998·Pharmaceutical Research·D E SmithJ B Schnermann
Apr 13, 1999·The American Journal of Physiology·M ThamotharanS A Adibi
Nov 13, 2001·American Journal of Physiology. Renal Physiology·K TakahashiK Inui
Mar 19, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kayoko AshidaKen-Ichi Inui
Mar 28, 2002·Diabetes·Ilan GabrielyNir Barzilai
Sep 12, 2002·Plant Physiology·B. ShenH. J. Bohnert
Dec 18, 2002·American Journal of Physiology. Endocrinology and Metabolism·Annette M Chang, Jeffrey B Halter
Jul 25, 2003·Journal of Molecular and Cellular Cardiology·Nicola KingM-Saadeh Suleiman
Aug 14, 2003·Experimental Gerontology·Klaus Turnheim
Oct 15, 2003·Archives of Surgery·Oliver O AalamiRandall P Nacamuli
Dec 18, 2003·British Journal of Clinical Pharmacology·A A Mangoni, S H D Jackson
May 25, 2004·The Journal of Biological Chemistry·Barbara MeissnerRalf Baumeister
Oct 6, 2005·Biological Chemistry·Frank DöringDavid A Groneberg
Jun 29, 2006·Annals of the New York Academy of Sciences·Robin Holliday
Oct 10, 2006·American Journal of Physiology. Renal Physiology·Gianfranco TramontiYashpal S Kanwar
Jan 4, 2007·The Journal of Pathology·J E Martin, M T Sheaff
Feb 20, 2007·Postgraduate Medical Journal·Elisabete Pinto
May 15, 2007·Clinical Pharmacology and Therapeutics·J B Schwartz
Jul 12, 2007·The Journal of Pharmacology and Experimental Therapeutics·Patrick HindletMarion Buyse
Jan 9, 2008·European Journal of Clinical Pharmacology·Shaojun ShiUlrich Klotz
Mar 25, 2008·Hormones : International Journal of Endocrinology and Metabolism·Robin P Peeters
Apr 18, 2008·The Journal of Pharmacy and Pharmacology·Matthias BrandschEva Bosse-Doenecke
May 27, 2008·Journal of the International Society of Sports Nutrition·Chad Kerksick, Darryn Willoughby
Jul 9, 2010·Advances in Chronic Kidney Disease·Jessica R Weinstein, Sharon Anderson
Oct 6, 2011·Archives of Pathology & Laboratory Medicine·Yan SunYiping Wang
Dec 13, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Abeer AbousaabFlorian Lang

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