Molecular chaperones are present in the thylakoid lumen of pea chloroplasts

FEBS Letters
T Schlicher, Jürgen Soll

Abstract

A soluble protein fraction was obtained from pea chloroplast thylakoids, which represents highly enriched lumenal components. Using antisera against chaperonin 60 (cpn60), chaperonin 10 (cpn10) and the heat shock cognate protein of 70 kDa (hsc70) we are able to demonstrate, that the thylakoid lumen contains a separate set of molecular chaperones, which is distinct from the stromal one. In contrast to the alpha and beta subunits of cpn60 present in the stroma the lumen contains only one cpn60 isoform of distinct isoelectric point. Furthermore the lumenal cpn10 is of 'normal' size and not like its stromal counterpart of a double-domain tandem architecture. The immunoreactive hsc70 isoforms in the lumen seem also to be different from the stromal ones. Thus, chloroplasts seem to contain the largest number of molecular chaperone isoforms present in one organelle.

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·U BertschP V Viitanen
Jan 2, 1992·Nature·M J Gething, J Sambrook
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T H LubbenP V Viitanen
Jul 28, 1995·The Journal of Biological Chemistry·P V ViitanenJ Soll
Oct 1, 1994·Plant Molecular Biology·C Robinson, R B Klösgen
Sep 1, 1994·Trends in Biochemical Sciences·N PfannerM Meijer
Feb 1, 1994·Trends in Biochemical Sciences·R A StuartW Neupert
Oct 1, 1993·Trends in Cell Biology·S High, C J Stirling

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Citations

Mar 8, 2011·Cellular and Molecular Life Sciences : CMLS·Shigeharu Sato
Nov 26, 2008·Plant Molecular Biology·Celeste WeissAbdussalam Azem
Aug 21, 2013·Photosynthesis Research·Radek Kaňa
Feb 19, 2009·Cell Stress & Chaperones·Anat L BonshtienCeleste Weiss
Jun 1, 2005·Molecular and Biochemical Parasitology·Shigeharu Sato, R J M Iain Wilson
Apr 17, 1999·The Plant Journal : for Cell and Molecular Biology·Y KoumotoM Nishimura
Mar 4, 2000·Cell Stress & Chaperones·R A Ivey, B D Bruce
Oct 16, 2001·Cell Stress & Chaperones·J E Hill, S M Hemmingsen
Jan 23, 2004·Proceedings of the National Academy of Sciences of the United States of America·Cornelia SpeteaBertil Andersson
Jun 23, 2015·Frontiers in Molecular Biosciences·Andrija FinkaPierre Goloubinoff
Jan 18, 2015·Biochimica Et Biophysica Acta·Raphael TröschFelix Willmund
Jun 5, 2008·Biochimica Et Biophysica Acta·Johannes M HerrmannH Ekkehard Neuhaus
Feb 7, 2018·Frontiers in Molecular Biosciences·Qian Zhao, Cuimin Liu
Apr 18, 1998·The Journal of Biological Chemistry·T KieselbachW P Schröder
Nov 24, 2001·The Journal of Biological Chemistry·Maria SchubertThomas Kieselbach
Jun 13, 2001·The Journal of Biological Chemistry·Y KoumotoM Nishimura
Feb 6, 2010·Molecular Biology Reports·Hong-Mei YuanJing Jiang
Dec 15, 2000·Biochimica Et Biophysica Acta·T M BrickerL K Frankel

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