Molecular Characterisation and Phylogenetic Analysis of a Novel Isoform of Hepatic Antimicrobial Peptide, Hepcidin (Zc-hepc1), from the Coral Fish Moorish idol, Zanclus cornutus (Linnaeus, 1758)

Probiotics and Antimicrobial Proteins
E R ChaithanyaI S Bright Singh

Abstract

Hepcidin is a family of short cysteine-rich antimicrobial peptides (AMPs) participating in various physiological functions with inevitable role in host immune responses. Present study deals with identification and characterisation of a novel hepcidin isoform from coral fish Zanclus cornutus. The 81 amino acid (aa) preprohepcidin obtained from Z. cornutus consists of a hydrophobic aa rich 22 mer signal peptide, a highly variable proregion of 35 aa and a bioactive mature peptide with 8 conserved cysteine residues which contribute to the disulphide back bone. The mature hepcidin, Zc-hepc1 has a theoretical isoelectric point of 7.46, a predicted molecular weight of 2.43 kDa and a net positive charge of +1. Phylogenetic analysis grouped Z. cornutus hepcidin with HAMP2 group hepcidins confirming the divergent evolution of hepcidin-like peptide in fishes. Zc-hepc1 can attain a β-hairpin-like structure with two antiparallel β-sheets. This is the first report of an AMP from the coral fish Z. cornutus.

References

Dec 13, 2000·The Journal of Biological Chemistry·C H ParkT Ganz
May 3, 2002·European Journal of Biochemistry·Hiroko ShikeJane C Burns
Apr 17, 2003·Developmental and Comparative Immunology·Susan E DouglasStephen C M Tsoi
Aug 22, 2003·Journal of Internal Medicine·H G Boman
Nov 24, 2004·International Journal of Antimicrobial Agents·K V R ReddyC Aranha
Jun 14, 2005·Fish & Shellfish Immunology·Bergljót Magnadóttir
Dec 6, 2005·Developmental and Comparative Immunology·Jishu Shi, Alvin C Camus
Apr 18, 2006·Developmental and Comparative Immunology·Pedro N S RodriguesJonathan M Wilson
Oct 2, 2007·Blood Cells, Molecules & Diseases·Erika V Valore, Tomas Ganz
Nov 21, 2007·Blood Cells, Molecules & Diseases·Erwin H J M KemnaDorine W Swinkels
Mar 4, 2008·Molecular Biology and Evolution·Qianghua XuLiangbiao Chen
Jul 2, 2008·Cell Metabolism·Martina U Muckenthaler
Jul 8, 2008·Fish & Shellfish Immunology·Terese SolstadTrond Ø Jørgensen
Jan 27, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Marco ScocchiRenato Gennaro
Mar 7, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Edward J NogaShannon Kozlowicz
Apr 3, 2009·Fish & Shellfish Immunology·Beatriz Martin-AntonioManuel Manchado
Aug 28, 2010·Molecular Immunology·Samuel T WorkenheFrederick S B Kibenge
Feb 16, 2011·Research in Microbiology·Gergely MarótiPeter Mergaert
Feb 22, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Bhawna SinghS K Gupta
Oct 1, 2011·Fish & Shellfish Immunology·Yoon Kwon NamDong Soo Kim
Oct 25, 2011·Fish & Shellfish Immunology·J V NevesP N S Rodrigues

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Citations

Dec 7, 2021·Journal of Biomolecular Structure & Dynamics·Wan-Atirah AzeminMohd Shahir Shamsir

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