PMID: 9426603Jan 14, 1998Paper

Molecular characterisation of a cDNA sequence encoding the backbone of a style-specific 120 kDa glycoprotein which has features of both extensins and arabinogalactan proteins

Plant Molecular Biology
C J SchultzA E Clarke

Abstract

Nicotiana alata has a style-specific hydroxyproline-rich glycoprotein (the 120 kDa glycoprotein) which has properties of both extensins and AGPs [19, 20]. The 120 kDa glycoprotein is a soluble component in the extracellular matrix of the transmitting tract of styles where it accounts for ca. 9% of the total buffer-soluble protein. Here we describe the molecular cloning of a cDNA representing the gene NaPRP5 which encodes the backbone of the 120 kDa glycoprotein. Expression of mRNA is restricted to styles, consistent with observations on the distribution of the 120 kDa glycoprotein. Levels of accumulation of the transcript encoding the 120 kDa protein backbone are not altered significantly by pollination with either compatible or incompatible pollen. The protein backbone of the 120 kDa glycoprotein, as predicted by the cDNA sequence, is composed of three distinct domains. The sequence of these domains, together with linkage analysis of the carbohydrate component of the 120 kDa glycoprotein, allows predictions of the likely distribution of substituent glycosyl chains along the protein backbone. The similarity of the C-terminal domains of the 120 kDa glycoprotein and GaRSGP, the galactose-rich style glycoprotein of N. alata, is co...Continue Reading

Citations

Aug 24, 2005·The Plant Journal : for Cell and Molecular Biology·C Nathan HancockBruce A McClure
Jul 2, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·C Nathan HancockBruce McClure
Jan 19, 2010·Plant & Cell Physiology·Tetsuya Higashiyama
Aug 2, 2011·Annals of Botany·Bruce McClureCarlos Romero
Jul 13, 2012·Annals of Botany·Eric Nguema-OnaAzeddine Driouich
Feb 7, 2015·Plant Reproduction·Ana Marta PereiraSílvia Coimbra
Jan 31, 2018·Journal of Cell Science·Yoko Mizuta, Tetsuya Higashiyama
Jan 22, 2005·The Plant Cell·Patrick J FerrisUrsula W Goodenough
Sep 1, 2001·The New Phytologist·M J WheelerF C H Franklin
Mar 20, 2003·Plant Physiology·Fung T LayMarilyn A Anderson
Apr 15, 2010·Plant Physiology·Miriam EllisAntony Bacic
Oct 19, 2004·Biochimica Et Biophysica Acta·Patricia GuzzardiElisabeth Jamet

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