Molecular characterisation of macrolide resistance mechanisms of Streptococcus pneumoniae and Streptococcus pyogenes isolated in Germany, 2002-2003

International Journal of Antimicrobial Agents
Ralf René ReinertAdnan Al-Lahham

Abstract

In the present study, a real-time PCR protocol was developed for the detection of macrolide resistance determinants and was validated in a nationwide study in Germany covering a total of 236 Streptococcus pyogenes and 241 Streptococcus pneumoniae strains collected from children < or = 16 years of age with community-acquired infections. Macrolide resistance was observed in 19.9% of pneumococcal strains and 14% of S. pyogenes isolates. Of the erythromycin A-resistant S. pyogenes strains, 93.9% showed the efflux type mef(A); 62.5% of the S. pneumoniae strains were mef(A)- and 37.5% erm(B)-positive. The correlation of the results of real-time PCR assay genotyping in the present study compared with those of conventional PCR genotyping and resistance phenotyping was 100%. Macrolide resistance is of growing concern in Germany. This highly sensitive and specific PCR assay to detect macrolide resistance has the potential to provide sufficiently rapid results to improve antibiotic treatment of streptococcal infections.

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Citations

Jan 9, 2009·Applied Microbiology and Biotechnology·Xu-Xiang ZhangHerbert H P Fang
Oct 27, 2006·Antimicrobial Agents and Chemotherapy·Adnan Al-LahhamRalf René Reinert
Mar 26, 2008·Antimicrobial Agents and Chemotherapy·Elia Gómez G de la PedrosaRafael Cantón
Jul 29, 2005·Antimicrobial Agents and Chemotherapy·Ralf René ReinertAdnan Al-Lahham
Apr 28, 2006·Antimicrobial Agents and Chemotherapy·P LittauerG S Simonsen
Sep 29, 2006·Journal of Clinical Microbiology·Mahbubur RahmanRalf René Reinert
Mar 8, 2005·Journal of Clinical Microbiology·Ralf René ReinertFranz-Josef Schmitz
Dec 17, 2009·PloS One·Mark van der LindenRalf René Reinert
Mar 23, 2011·International Journal of Antimicrobial Agents·Christine BleyRalf René Reinert
Sep 3, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C PlainvertUNKNOWN CNR-Strep network
Mar 12, 2005·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C NeelemanJ W Mouton
Mar 16, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·R R ReinertR von Kries
Feb 12, 2005·The Journal of Antimicrobial Chemotherapy·Rafael CantónGiuseppe Cornaglia
Mar 1, 2006·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Cord SunderkötterUta Jappe
Mar 10, 2015·Expert Review of Anti-infective Therapy·Catarina Silva-CostaJose Melo-Cristino
Jul 11, 2014·Indian Journal of Medical Microbiology·W ZhouC M Wa

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