Molecular characterisation of multidrug-resistant Mycobacterium tuberculosis isolates from a high-burden tuberculosis state in Brazil

Epidemiology and Infection
R S SalvatoMaria Lucia R Rossetti

Abstract

Tuberculosis (TB) is the leading cause of death among infectious diseases worldwide. Among the estimated cases of drug-resistant TB, approximately 60% occur in the BRICS countries (Brazil, Russia, India, China and South Africa). Among Brazilian states, primary and acquired multidrug-resistant TB (MDR-TB) rates were the highest in Rio Grande do Sul (RS). This study aimed to perform molecular characterisation of MDR-TB in the State of RS, a high-burden Brazilian state. We performed molecular characterisation of MDR-TB cases in RS, defined by drug susceptibility testing, using 131 Mycobacterium tuberculosis (M.tb) DNA samples from the Central Laboratory. We carried out MIRU-VNTR 24loci, spoligotyping, sequencing of the katG, inhA and rpoB genes and RDRio sublineage identification. The most frequent families found were LAM (65.6%) and Haarlem (22.1%). RDRio deletion was observed in 42 (32%) of the M.tb isolates. Among MDR-TB cases, eight (6.1%) did not present mutations in the studied genes. In 116 (88.5%) M.tb isolates, we found mutations associated with rifampicin (RIF) resistance in rpoB gene, and in 112 isolates (85.5%), we observed mutations related to isoniazid resistance in katG and inhA genes. An insertion of 12 nucleotides...Continue Reading

References

Jul 28, 2006·Antimicrobial Agents and Chemotherapy·Manzour Hernando HazbónDavid Alland
Jul 13, 2007·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Suzana DavidJosé Germano de Sousa
Sep 28, 2007·Journal of Clinical Microbiology·Luiz Claudio Oliveira LazzariniJohn L Ho
May 9, 2008·Journal of Clinical Microbiology·Luiz Claudio Oliveira LazzariniJohn L Ho
Sep 20, 2008·PLoS Pathogens·Thierry WirthStefan Niemann
Nov 16, 2010·Drug Discovery Today. Disease Mechanisms·Mireilla Coscolla, Sebastien Gagneux
Mar 4, 2011·Future Microbiology·Midori Kato-MaedaLaura Flores
Aug 13, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Scott A WeisenbergJohn L Ho
Oct 18, 2011·Tuberculosis·Paulo F PerizzoloMaria Lucia R Rossetti
Dec 16, 2011·Journal of Clinical Microbiology·Jessica L de BeerUNKNOWN Global Network for the Molecular Surveillance of Tuberculosis 2009
Dec 27, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Russell R KempkerSergo Vashakidze
Feb 9, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Sebastien Gagneux
Aug 7, 2012·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·C de B BarbosaN Boéchat
Dec 3, 2014·Seminars in Immunology·Mireia Coscolla, Sebastien Gagneux
Jul 15, 2015·Memórias do Instituto Oswaldo Cruz·Rodrigo Ivan PrimMaria Luiza Bazzo
Oct 23, 2016·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·D PapaventsisV Nikolayevskyy
Feb 22, 2017·PloS One·Christelle VluggenVanessa Mathys
Mar 24, 2017·The European Respiratory Journal·Mayara Lisboa BastosDick Menzies
May 11, 2017·Frontiers in Microbiology·Mohammad J NasiriMohammad M Feizabadi
Feb 20, 2018·Nature Reviews. Microbiology·Sebastien Gagneux
Mar 22, 2018·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·João PerdigãoIsabel Portugal
Mar 23, 2018·Infection and Drug Resistance·Guisheng XuHongqiu Pan

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Citations

Aug 1, 2020·Scientific Reports·Mirela VerzaMaria Luiza Bazzo
Mar 18, 2021·Memórias do Instituto Oswaldo Cruz·Emilyn Costa ConceiçãoKarla Valéria Batista Lima
Jul 22, 2021·International Journal of Antimicrobial Agents·Richard Steiner SalvatoMaria Lucia Rosa Rossetti
Nov 30, 2019·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Richard Steiner SalvatoMaria Lucia Rosa Rossetti
Aug 29, 2020·Enfermedades infecciosas y microbiología clínica·Virgilio Bocanegra-GarcíaJosé Prisco Palma-Nicolás

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Methods Mentioned

BETA
genotyping
X-ray

Software Mentioned

Chromas
Blast
SeqScape
Genemapper

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