Molecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facility in the state of Amazonas-Brazil

Mycoses
Ana Karla FreireJoão Vicente De Souza

Abstract

As there are four major molecular types of Cryptococcus neoformans (VNI, VNII, VNIII and VNIV) and four molecular types of Cryptococcus gattii (VGI, VGII, VGIII and VGIV), it is important to identify the specific groups causing cryptococcosis in different geographical regions. Here, we investigated the molecular types of 57 cryptococcal isolates from patients in a tertiary care hospital in the state of Amazonas, Brazil, between 2006 and 2010. The isolates were characterised by PCR fingerprinting using the M13 minisatellite and confirmed by URA5-RFLP analysis, and the presence of specific genes from the mating type locus (MATα and MATa) of these species was analysed by PCR. Most of the patients were male (66.7%), between 16 and 30 years of age (51.7%), and HIV-positive (75.0%). Most isolates were collected from cerebrospinal fluid samples (71.7%). Most of the C. neoformans isolates (n=40) were characterised as members of the VNI molecular group (n=39), a unique isolate was characterised as VNII whereas all isolates of C. gattii (n=17) were members of the VGII molecular group. With regard to mating types, 55 isolates were type 'α', and only two were type 'a'. This study revealed the prevalence of the VNI molecular group and provi...Continue Reading

References

Mar 1, 1997·Journal of Clinical Microbiology·C Lo PassoA Pernice
Sep 23, 1997·Seminars in Cutaneous Medicine and Surgery·F M Durden, B Elewski
Feb 27, 2003·Emerging Infectious Diseases·Wieland MeyerUNKNOWN IberoAmerican Cryptococcal Study Group
May 16, 2003·FEMS Yeast Research·Agnes Kiesling CasaliMarilene Henning Vainstein
Jun 6, 2003·Microbes and Infection·Judith N Steenbergen, Arturo Casadevall
Dec 2, 2004·Proceedings of the National Academy of Sciences of the United States of America·S E KiddW Meyer
Mar 15, 2008·Revista iberoamericana de micología·Marjan BoversTeun Boekhout
Mar 15, 2008·Revista iberoamericana de micología·Josep M Torres-RodríguezRicardo Gutiérrez-Gallego
Sep 18, 2008·Memórias do Instituto Oswaldo Cruz·Luciana TrillesWieland Meyer
Jan 17, 2009·Memórias do Instituto Oswaldo Cruz·Wallace Raimundo A dos SantosMárcia dos Santos Lazéra
Aug 22, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S-J LiawP-R Hsueh
Jul 20, 2010·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M S SantosJ V B Souza

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Citations

Nov 26, 2014·Mycoses·María T Illnait-ZaragoziJacques F Meis
Feb 28, 2015·Fungal Genetics and Biology : FG & B·Ferry HagenTeun Boekhout
May 3, 2016·Mycoses·Franqueline Reichert-LimaAngelica Z Schreiber
Aug 2, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·P F HerkertF Queiroz-Telles
Oct 16, 2014·Memórias do Instituto Oswaldo Cruz·Jairo LizarazoElizabeth Castañeda
Jul 13, 2013·Mycopathologia·Rosianne Assis de Sousa TsujisakiMarilene Rodrigues Chang
Jan 27, 2018·Journal of Fungi·Patricia F HerkertFlávio Queiroz-Telles
Mar 11, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Amaury Dos Santos BentesJoão Vicente Braga de Souza
Apr 12, 2018·Memórias do Instituto Oswaldo Cruz·Carolina FiracativeUNKNOWN Latin American Cryptococcal Study Group

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