Molecular characteristic of Pasteurella multocida isolates from Sumba Island at East Nusa Tenggara Province, Indonesia

Veterinary World
I K NarcanaI N K Besung

Abstract

This study aimed to determine the molecular characteristics of Pasteurella multocida isolates originated from Sumba Island, East Nusa Tenggara Province. The isolates of P. multocida stored in frozen storage were cultured in blood agar as a selective medium and identified conventionally. Molecular tests were initiated by DNA isolation and then followed by polymerase chain reaction tests with specific primers for the determination of P. multocida serotype A or B. Positive strain of serotype B was then confirmed molecularly using 16S rRNA gene primer and followed by the sequencing of nucleotides. The study showed that both P. multocida isolates from Sumba island, i.e. PM1 is isolated from East Sumba district, while PM2 isolated from West Sumba district have 99.6% homology. Both isolates also known have 99% similarities with P. multocida originated from India, Britain, and Japan, respectively. The isolates share the same clade in the phylogenetic tree. The 16S rRNA sequencing revealed a high similarity of P. multocida serotype B:2 isolated from Sumba island with the Indian isolates although the sample size is very small. Therefore, further molecular studies like multilocus sequence typing, VNTR need to be performed using a larger n...Continue Reading

References

May 23, 1998·Journal of Clinical Microbiology·K M TownsendH J Dawkins
Nov 17, 2006·FEMS Microbiology Letters·Marina HarperBen Adler
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Mar 1, 2012·Current Topics in Microbiology and Immunology·Marina HarperBen Adler
May 31, 2012·Current Topics in Microbiology and Immunology·I W WilkieB Adler
Sep 12, 2014·Transboundary and Emerging Diseases·L N SarangiV P Singh
Jun 19, 2016·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·I Wayan SuardanaBudi Setiadi Daryono
May 17, 2017·Acta Tropica·I Wayan SuardanaI Nyoman Suyasa

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Datasets Mentioned

BETA
DQ286927
AY078999
KT222136
AY638485

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

MEGA
BLAST

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