Abstract
Heat shock proteins play essential roles in basic cellular events. Spawning migration is a complex process, with significant structural and biochemical changes taking place in the adult gonad. To date, the molecular mechanisms underlying migration reproductive biology remain undetermined. In this regard, a full length HSP90AA1 comprising 2608 nucleotides from the anadromous fish Coilia nasus was characterized, encoding 742 amino acid (aa) residues with potential phosphorylation sites. HSP90AA1 mRNA transcripts were detected in all organs, especially in the gonad. Furthermore, the greatest transcript levels were found during the developmental phase, while the lowest levels were found during the resting phase. In addition, the strongest immunolabeling positive signal was found in the primary spermatocyte and oocyte, with lower positive staining in secondary germ cells, and a weak or absent level in the mature sperm and oocyte. Interestingly, HSP90AA1 was mainly located in the cytoplasm of germ cells. These results are important for understanding the molecular mechanism of anadromous migration reproductive biology. In combination with data from other fish species, the result of this present study may facilitate further investigati...Continue Reading
References
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Sep 28, 1998·Pharmacology & Therapeutics·P CsermelyG Nardai
May 14, 1999·Trends in Biochemical Sciences·J Buchner
Dec 20, 2002·Angewandte Chemie·Stefan Walter, Johannes Buchner
Jan 24, 2004·Reviews of Physiology, Biochemistry and Pharmacology·H WegeleJ Buchner
Nov 17, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Thomas P Mommsen
Jun 8, 2006·Annual Review of Biochemistry·Laurence H Pearl, Chrisostomos Prodromou
May 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Frédéric ChalmelMichael Primig
Sep 11, 2007·Biochimie·Manolo Gouy, Stéphane Delmotte
Oct 9, 2007·Methods : a Companion to Methods in Enzymology·Gabriele Multhoff
Apr 8, 2009·General and Comparative Endocrinology·Rüdiger W SchulzTakeshi Miura
May 9, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Nathalie BissonnetteGuylain Boissonneault
Jun 10, 2009·General and Comparative Endocrinology·Esther LubzensJoan Cerdà
Aug 6, 2010·Progress in Neurobiology·R Anne StetlerJun Chen
Feb 26, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Janet GenzMartin Grosell
May 25, 2011·Biochimica Et Biophysica Acta·Ashraf A KhalilChristopher Smith
Dec 5, 2012·Journal of Signal Transduction·Takashi W IjiriKen-Ichi Sato
Jun 26, 2013·Cell Reports·Magali SoumillonHenrik Kaessmann
Nov 30, 2013·Pharmaceuticals·Nathan LawlessGennady Verkhivker
Jan 7, 2014·Biochimica Et Biophysica Acta·Alejandra G ErlejmanMario D Galigniana
Oct 2, 2014·Fish & Shellfish Immunology·Dongxue XuHongsheng Yang
Jan 13, 2015·Cryobiology·Xiao-Gang ZhangJian-Hong Hu
Mar 4, 2015·Marine Genomics·Jin-Rong DuanDi-An Fang
Mar 17, 2015·Seminars in Cell & Developmental Biology·Heather L SmithMichael E Cheetham
Jun 27, 2015·Marine Genomics·Yan-Feng ZhouPao Xu
Citations
Jul 30, 2017·Genes·Di-An FangJin-Rong Duan
Sep 19, 2017·Annals of Clinical Biochemistry·Eiji MunetsunaKoji Suzuki
Aug 11, 2020·Heliyon·Ly SoktaJian Yang
Jan 26, 2021·Bioscience Reports·Lei GaoShuqiu Zhang