Molecular Characteristics of "BlaB-Like" Chromosomal Inducible Cephalosporinase of Yersinia enterocolitica Biotype 1A Strains

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
Neelja SinghalJugsharan S Virdi

Abstract

Yersinia enterocolitica biotype 1A strains are emerging pathogens, frequently isolated from clinical samples across the globe. Y. enterocolitica strains produce two chromosomal β-lactamases, BlaA and BlaB. BlaA is a constitutively expressed, Ambler class A, penicillinase, whereas BlaB is Ambler class C-type, inducible cephalosporinase. An earlier study from our laboratory indicated that instead of BlaB, Y. enterocolitica biotype 1A produced a "BlaB-like" enzyme. The objective of this work was to study the molecular characteristics of "Bla-B like" β-lactamases produced by biotype 1A strains to discern their similarity with AmpC-type β-lactamases and the basis of varied levels of minimum inhibitory concentration (MIC) to cefotaxime. Thus, the promoters and blaB genes were investigated in four strains of biotype 1A. Three-dimensional structures of the "BlaB-like" enzymes were modeled, and docked in silico with cefotaxime to understand how specific substitutions in gene sequences affect antibiotic susceptibilities. Our results indicated that all the reported key catalytic residues were present in variants of "Bla-B-like" enzymes, discerned in biotype 1A strains, but at different positions. Molecular docking of enzyme variants with ...Continue Reading

References

May 1, 1992·Antimicrobial Agents and Chemotherapy·A SeoaneJ M García Lobo
Mar 5, 1992·Nature·R LüthyD Eisenberg
Sep 1, 1993·Protein Science : a Publication of the Protein Society·C Colovos, T O Yeates
Apr 1, 1997·Clinical Microbiology Reviews·E J Bottone
Oct 27, 1999·Journal of Medical Microbiology·I StockB Wiedemann
May 8, 2000·Journal of Medical Microbiology·I StockB Wiedemann
Jun 23, 2001·The Journal of Antimicrobial Chemotherapy·D M Livermore, D F Brown
Jul 23, 2004·The Journal of Antimicrobial Chemotherapy·Sachin SharmaJ S Virdi
Mar 2, 2006·Journal of the American Chemical Society·Yu ChenBrian K Shoichet
Mar 24, 2006·FEMS Microbiology Letters·Sachin SharmaJugsharan S Virdi
Jan 13, 2009·Clinical Microbiology Reviews·George A Jacoby
Oct 5, 2010·Critical Reviews in Microbiology·Neeru Bhagat, Jugsharan S Virdi
Jan 24, 2014·Bioinformatics·Philip JonesSarah Hunter
May 21, 2016·Journal of Applied Microbiology·E Terech-MajewskaK Grabowska

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

PROCHECK
BLAST
Interproscan
PyMoL
MODELLER
Primer
Verify
Verify 3D
AutoDock
AutoDockTools ( ADT )

Related Concepts

Related Feeds

CRISPR Screens in Drug Resistance

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of CRISPR-Cas system in high-throughput genome-wide screens to identify genes that may confer drug resistance.