PMID: 8597566Feb 8, 1996Paper

Molecular characteristics of dimethylnitrosamine induced fibrotic liver collagen

Biochimica Et Biophysica Acta
J George, G Chandrakasan

Abstract

The molecular characteristics of purified pepsin solubilized collagen from rat liver was studied in control and dimethylnitrosamine administered animals. The alpha- and beta-chains of purified pepsin solubilized liver collagen were separated by subjecting the denatured collagen to SDS-polyacrylamide gel electrophoresis. The alpha 1(III) chains were resolved from the alpha 1(I) chains by interrupted electrophoresis with delayed reduction of the disulfide bonds of type III collagen. The aldehyde content of the purified pepsin solubilized collagen was estimated in control and experimental samples in order to assess the extent of collagen cross-links. Fibril formation curves were studied with purified pepsin solubilized collagen to see the rate of formation of cross-links within the fibrillar mesh. The results of the unreduced electrophoretic studies revealed a significant increase in the beta-subunit of type I collagen with a remarkable decrease of alpha/beta ratio in DMN treated animals. Reduction with beta-mercaptoethanol indicated the presence of type III collagen in the electrophoretic field with a proportionate increase on the 21st day. A significant increase in the aldehyde content and an increased rate of fibril formation w...Continue Reading

References

Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R C SiegelJ M Aguiar
Jul 1, 1978·The American Journal of Clinical Nutrition·R B Rucker, J Murray
Jan 1, 1979·International Review of Connective Tissue Research·R C Siegel
Feb 1, 1976·Agents and Actions·S BazinA Delaunay
Oct 18, 1976·Biochemical and Biophysical Research Communications·B SykesR Smith
Feb 1, 1977·The Journal of Clinical Investigation·J M SeyerA H Kang
Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·M Rojkind, A Martinez-Palomo
Mar 8, 1976·Biochemical and Biophysical Research Communications·K Fujii, M L Tanzer
May 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P D HallM J Cousins
Jan 1, 1989·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·F AriostoL Capocaccia
Jan 1, 1989·Journal of Hepatology·A M JézéquelF Orlandi
Jan 1, 1985·Journal of Hepatology·S A JenkinsR Shields
Jan 1, 1972·Annual Review of Biochemistry·P M GallopS Seifter
Aug 1, 1983·Seminars in Arthritis and Rheumatism·M E Nimni
Jul 1, 1983·Collagen and Related Research·M RojkindJ Cordero-Hernández
Jan 1, 1984·Methods in Enzymology·P J Blackshear
Mar 1, 1965·Archives of Biochemistry and Biophysics·M A PAZP M GALLOP
Jun 9, 2004·Journal of the History of Medicine and Allied Sciences·John C Waller
Dec 5, 2013·Genetics and Molecular Research : GMR·P K YangX T Kang

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Citations

Jun 7, 2011·Pharmaceutical Research·Ravi S ShuklaKun Cheng
Aug 21, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Joseph George
Dec 4, 2003·The International Journal of Biochemistry & Cell Biology·Joseph GeorgeShujiro Takase
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·S DeltourD Leprince
May 26, 2012·Saudi Journal of Gastroenterology : Official Journal of the Saudi Gastroenterology Association·Areeba Ahmad, Riaz Ahmad
Sep 10, 2014·Life Sciences·Dania AkbikRamin Rohanizadeh
Jul 11, 2009·Chemico-biological Interactions·Riaz AhmadAbsar-ul Hasnain
Sep 17, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Joseph George, Robert Stern
Sep 19, 2012·Environmental Toxicology and Pharmacology·Devaraj EzhilarasanPalani Vivekanandan
Sep 9, 2006·Clinical Biochemistry·Joseph George
Jun 5, 2008·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Joseph George
Aug 7, 2017·Journal of Cellular and Molecular Medicine·Joseph GeorgeMutsumi Tsuchishima
Aug 8, 2006·Tissue Engineering·L YaoR M Aspden
Jan 11, 2014·Pharmaceutical Biology·Areeba AhmadRiaz Ahmad
Oct 25, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Keon Wook KangSang Geon Kim
Jul 16, 2020·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Joseph GeorgeMikihiro Tsutsumi
Jan 27, 2019·Scientific Reports·Joseph GeorgeMutsumi Tsuchishima
Jan 10, 2019·Cell Death & Disease·Joseph GeorgeMikihiro Tsutsumi
Jun 19, 2001·Biochimica Et Biophysica Acta·B MuguerzaJ Prieto
Jun 15, 2021·Frontiers in Physiology·Yu-Long BaoShao-Wei Li
Sep 6, 2012·Molecular Pharmaceutics·Chandana MohantySanjeeb K Sahoo

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