PMID: 2253484Jan 1, 1990

Molecular characteristics of pepsinogen and pepsin from duck glandular stomach

Comparative Biochemistry and Physiology. B, Comparative Biochemistry
I Pichová, V Kostka

Abstract

1. Two procedures were developed for the preparation of duck pepsinogen, an enzyme from the family of aspartic proteases (EC 3.4.23.1) and its zymogen. 2. The amino acid composition, sugar content and the partial N- and C-terminal sequences of both the enzyme and the zymogen were determined. These sequences are highly homologous with the terminal sequences of chicken pepsin(ogen). 3. Duck pepsinogen and pepsin are unlike other pepsin(ogen)s in being relatively stable in alkaline media: pepsinogen is inactivated at pH 12.1, pepsin at pH 9.6. 4. Duck pepsin is inhibited by diazoacetyl-D,L-norleucine methyl ester (DAN), 1,2-epoxy-3(p-nitrophe-noxy)propane (EPNP), pepstatin and a synthetic pepsin inhibitor Val-D-Leu-Pro-Phe-Phe-Val-D- Leu. The pH-optimum of duck pepsin determined in the presence of synthetic substrate is pH 4. 5. Duck pepsin has a marked milk-clotting activity whereas its proteolytic activity is lower than that of chicken pepsin. 6. The activation of duck pepsinogen is paralleled by two conformational changes. The activation half-life determined in the presence of a synthetic substrate at pH 2 and 14 degrees C is 20 sec.

References

Dec 1, 1995·The International Journal of Biochemistry & Cell Biology·B I PletschkeW Oelofsen

Citations

Jun 1, 1976·Analytical Biochemistry·T H Plummer
Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·E StreicherW Oelofsen
Jul 1, 1974·Analytical Biochemistry·B PenkeK Kovács
Oct 17, 1983·European Journal of Biochemistry·M Baudys, V Kostka
Aug 1, 1965·Analytical Chemistry·J V BENSON, J A PATTERSON
Sep 20, 1932·The Journal of General Physiology·M L Anson, A E Mirsky
May 20, 1938·The Journal of General Physiology·R M HerriottJ H Northrop

Related Concepts

C1S
Pepsinogen B
Carboxy-Terminal Amino Acid
Pepsinogen Measurement
Pepsinogen A
Peptide Hydrolases
Chromatography, DEAE-Cellulose
Endopeptidases
Pepsin 3
Proteolytic Enzyme

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