PMID: 2253484Jan 1, 1990

Molecular characteristics of pepsinogen and pepsin from duck glandular stomach

Comparative Biochemistry and Physiology. B, Comparative Biochemistry
I Pichová, V Kostka


1. Two procedures were developed for the preparation of duck pepsinogen, an enzyme from the family of aspartic proteases (EC and its zymogen. 2. The amino acid composition, sugar content and the partial N- and C-terminal sequences of both the enzyme and the zymogen were determined. These sequences are highly homologous with the terminal sequences of chicken pepsin(ogen). 3. Duck pepsinogen and pepsin are unlike other pepsin(ogen)s in being relatively stable in alkaline media: pepsinogen is inactivated at pH 12.1, pepsin at pH 9.6. 4. Duck pepsin is inhibited by diazoacetyl-D,L-norleucine methyl ester (DAN), 1,2-epoxy-3(p-nitrophe-noxy)propane (EPNP), pepstatin and a synthetic pepsin inhibitor Val-D-Leu-Pro-Phe-Phe-Val-D- Leu. The pH-optimum of duck pepsin determined in the presence of synthetic substrate is pH 4. 5. Duck pepsin has a marked milk-clotting activity whereas its proteolytic activity is lower than that of chicken pepsin. 6. The activation of duck pepsinogen is paralleled by two conformational changes. The activation half-life determined in the presence of a synthetic substrate at pH 2 and 14 degrees C is 20 sec.


Dec 1, 1995·The International Journal of Biochemistry & Cell Biology·B I PletschkeW Oelofsen


Jun 1, 1976·Analytical Biochemistry·T H Plummer
Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·E StreicherW Oelofsen
Jul 1, 1974·Analytical Biochemistry·B PenkeK Kovács
Oct 17, 1983·European Journal of Biochemistry·M Baudys, V Kostka
Aug 1, 1965·Analytical Chemistry·J V BENSON, J A PATTERSON
Sep 20, 1932·The Journal of General Physiology·M L Anson, A E Mirsky
May 20, 1938·The Journal of General Physiology·R M HerriottJ H Northrop

Related Concepts

Pepsinogen B
Carboxy-Terminal Amino Acid
Pepsinogen Measurement
Pepsinogen A
Peptide Hydrolases
Chromatography, DEAE-Cellulose
Pepsin 3
Proteolytic Enzyme

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

© 2020 Meta ULC. All rights reserved