Molecular Characterization and Antimicrobial Activity of an Endolichenic Fungus, Aspergillus sp. Isolated from Parmelia caperata of Similipal Biosphere Reserve, India

Interdisciplinary Sciences, Computational Life Sciences
Srichandan PadhiKumananda Tayung

Abstract

Endolichenic fungi are microbes that inhabit healthy inner lichen tissues without any disease symptoms. They have been reported to produce new and interesting bioactive metabolites. In the present study, an endolichenic fungus frequently isolated from surface-sterilized lichen thallus of Parmelia caperata has been described. The fungus was identified as Aspergillus tubingensis based on morphological traits and ITS rDNA sequence. Crude metabolites extracted from the culture broth exhibited considerable antimicrobial activity against a panel of clinically significant human pathogens. The fungus showed optimum antimicrobial activity in PDB medium in day 7 of incubation period. PDB medium amended with 1 % NaCl and at alkaline pH was found to be optimal for antimicrobial metabolites production. Enhanced activity was observed when the fungus was exposed briefly to a heat shock of 60 °C during incubation. The metabolites showed optimum λ-max at 214 nm with an absorbance value of 1.589. Molecular characterization of the isolate was carried out by ITS phylogeny and ITS2 secondary structure analyses. The phylogenetic trees based on both ITS rDNA and ITS2 sequences showed the isolate within the clade A. tubingensis. Considering the ubiqui...Continue Reading

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Citations

Oct 22, 2019·Mycology·Abdulkawi Ali Al-Fakih, Wael Qasem Abdulgabbar Almaqtri
Apr 5, 2017·Interdisciplinary Sciences, Computational Life Sciences·Tayebeh FarhadiRoman S Ovchinnikov
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·A Nethma WethalawePriyani A Paranagama

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