Molecular characterization and diversity analysis of bacterial communities associated with Dialeurolonga malleswaramensis (Hemiptera: Aleyrodidae) adults using 16S rDNA amplicon pyrosequencing and FISH

Insect Science
Neeti Pandey, Raman Rajagopal

Abstract

Dialeurolonga malleswaramensis Sundararaj (Hemiptera: Aleyrodidae) is a phytophagous sap sucking insect. It infests Polyalthia longifolia, an important avenue tree of India, effective in alleviating noise pollution and having immense medicinal importance. Samples of this insect were collected from Polyalthia longifolia. The cytochrome c oxidase subunit I gene (mtCO1) helped in the molecular characterization of the insect. This study reports the bacterial diversity in D. malleswaramensis adults by high throughput 16S rDNA amplicon pyrosequencing. The major genera identified were Portiera and Arsenophonus. Other bacterial genera detected were uncultured alpha proteobacterium, Sphingopyxis and Methylobacterium. We also employed fluorescence in situ hybridization (FISH) in whole mount samples to confirm the presence of dominant endosymbionts Portiera and Arsenophonus to the bacteriocyte of D. malleswaramensis. This study concludes that combining techniques like 16S rDNA amplicon pyrosequencing and FISH reveal both dominant and rare bacteria. The data also predict the evolutionary position of this pest with respect to other whitefly species using a mitochondrial marker.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Dec 24, 1997·Genomics·W R PearsonW Miller
Jul 6, 2000·Applied and Environmental Microbiology·T FukatsuR Koga
Dec 4, 2003·Annual Review of Entomology·R J Dillon, V M Dillon
Jun 9, 2004·Applied and Environmental Microbiology·MyLo Ly Thao, Paul Baumann
Apr 16, 2005·Science·Paul B EckburgDavid A Relman
Feb 2, 2006·Journal of Experimental Botany·A E Douglas
May 5, 2006·Applied and Environmental Microbiology·Yuval GottliebEinat Zchori-Fein
Aug 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Mitchell L SoginGerhard J Herndl
Jun 26, 2007·Applied and Environmental Microbiology·Qiong WangJames R Cole
Feb 21, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yuval GottliebEinat Zchori-Fein
May 24, 2008·Science·Ruth E LeyJeffrey I Gordon
Oct 13, 2010·Proceedings of the National Academy of Sciences of the United States of America·Andrew K BensonDaniel Pomp
Jun 1, 2009·Indian Journal of Microbiology·R Rajagopal
Aug 31, 2013·PloS One·Matthew J MorganChristopher M Hardy
Dec 20, 2013·Molecular Ecology·Xiangfeng JingAngela E Douglas

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Citations

Oct 19, 2017·FEMS Microbiology Ecology·Neeti Pandey, Raman Rajagopal
Oct 2, 2019·Environmental Microbiology Reports·Ailsa H C McLeanLee M Henry
Oct 16, 2018·Frontiers in Microbiology·Diego Santos-GarciaFrancisco J Silva

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