Molecular characterization and exclusion of porcine GUSB as a candidate gene for congenital hernia inguinalis/scrotalis.

BMC Veterinary Research
Julia BeckBertram Brenig

Abstract

Inguinal hernias are usually caused by a congenital defect, which occurs as a weakness of the inguinal canal. Porcine beta-glucuronidase gene (GUSB) was chosen as functional candidate gene because of its involvement in degradation of hyaluronan within gubernacular tissue during descent of testes. Since a genome-wide linkage analysis approach has shown evidence that two regions on porcine chromosome 3 (SSC 3) are involved in the inheritance of hernia inguinalis/scrotalis in German pig breeds, GUSB also attained status as a positional candidate gene by its localization within a hernia-associated chromosomal region. A contig spanning 17,157 bp, which contains the entire GUSB, was assembled. Comparative sequence analyses were conducted for the GUSB gene locus. Single nucleotide polymorphisms (SNPs) located within the coding region of GUSB were genotyped in 512 animals. Results of transmission disequilibrium test (TDT) for two out of a total of five detected SNPs gave no significant association with the outcome of hernia in pigs. On the basis of our studies we are able to exclude the two analyzed SNPs within the porcine GUSB gene as causative for the transmission of inguinal hernia.

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Citations

Jan 16, 2009·Reproduction in Domestic Animals = Zuchthygiene·Y LiX Chen

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Datasets Mentioned

BETA
DQ095863
J02836
DQ095836
AC073261

Methods Mentioned

BETA
electrophoresis
genotyping
PCR

Software Mentioned

Genescan
Sequencher
Genotyper
RepeatMasker
BLAST
PiPMaker
CpG plot
[UNK]
Allegro
GASSOC

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