PMID: 2493648Mar 1, 1989Paper

Molecular characterization and expression in Escherichia coli of the gene complex encoding the polysaccharide capsule of Neisseria meningitidis group B

Proceedings of the National Academy of Sciences of the United States of America
Matthias FroschT F Meyer

Abstract

The gene complex encoding all determinants of the biosynthesis pathway of the capsule of group B meningococci (cps) has been cloned in Escherichia coli. A 24-kilobase large chromosomal fragment is necessary for capsule expression on the E. coli surface. By transposon and deletion mutagenesis, two separate steps in transport of the polysaccharide from the cytoplasm to the periplasm and further to the cell surface became evident. Mutants were also isolated that accumulate soluble poly(sialic acid) in the cytoplasm. The cloned cps complex conferred to E. coli strain GC6 sensitivity for E. coli K1-specific phages; phage sensitivity was enhanced in two distinct classes of cps mutants. Southern blot experiments revealed homology to some or all other Neisseria meningitidis capsular types and other Neisseria species, depending on the fragment of the cps complex used as probe.

References

Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A PoustkaH Lehrach

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Citations

Jan 1, 1988·Antonie van Leeuwenhoek·T F MeyerJ P van Putten
Mar 30, 2010·Applied Microbiology and Biotechnology·Miguel Angel Ferrero, Leandro Rodríguez Aparicio
Jan 1, 1991·Transactions of the Royal Society of Tropical Medicine and Hygiene·J M GriffissJ J Kim
Dec 15, 1992·FEMS Microbiology Letters·G BoulnoisI Roberts
Jul 8, 2000·Microbes and Infection·Y L Tzeng, D S Stephens
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·C R Tinsley, X Nassif
Sep 2, 2011·The Journal of Infectious Diseases·Ana Belén Ibarz-PavónMartin C J Maiden
Jun 27, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Martin C J Maiden
Apr 1, 1990·Molecular Microbiology·S M Steenbergen, E R Vimr
Jun 1, 1991·Molecular Microbiology·J S KrollE R Moxon
May 2, 2007·Infection and Immunity·Maria Rita SpinosaCecilia Bucci
May 23, 2003·Infection and Immunity·Aloka B BandaraThomas J Inzana
Feb 1, 2011·Journal of Bacteriology·Dwight C PetersonWillie F Vann
Feb 23, 2010·Journal of Bacteriology·Deborah M Tobiason, H Steven Seifert
Aug 21, 2012·Journal of Bacteriology·Lavanya RishishwarI King Jordan
Dec 18, 2002·Journal of Bacteriology·Guido DietrichMatthias Frosch
Aug 4, 2005·Journal of Bacteriology·Alexey TeplyakovGary L Gilliland
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May 1, 2013·Emerging Infectious Diseases·Odile B HarrisonMartin C J Maiden
Feb 17, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Linda M N HoangRusung Tan
Jan 7, 1997·Proceedings of the National Academy of Sciences of the United States of America·J S SwartleyD S Stephens
Jul 24, 2012·Current Opinion in Chemical Biology·Joshua T KittlesonJ Christopher Anderson
Jul 3, 2007·Lancet·David S StephensPetter Brandtzaeg

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