Molecular characterization and expression of p63 isoforms in human keloids

Molecular Genetics and Genomics : MGG
B De FeliceC Pinelli

Abstract

Keloids are benign skin tumors that develop following wounding. A cDNA product from human keloid specimens was identified using the differential display technique. The full-length cDNA was cloned by RT-PCR using human keloid mRNA as template. The predicted product of the cDNA was found to be 99% identical to the DeltaN-p63 gamma isotype of p63, a transcription factor that belongs to the family that includes the structurally related tumor suppressor p53 and p73. The DeltaN-p63 isotype lacks the acidic N terminal region corresponding to the transactivation domain of p53. Since this can potentially block p53-mediated target gene transactivation, it may serve as a dominant-negative isoform. Real-Time RT-PCR analysis of RNAs from normal skin tissue and keloids showed that the DeltaN-p63 isotype is specifically expressed in keloids, but is virtually undetectable in normal skin. Immunostaining of p63 in normal skin revealed that only basal cells of the epithelium expressed the protein, while in keloid tissues the antigen was detected in the nuclei of cells scattered through all layers of the epithelium and in fibroblast-like cells in the dermis. These results may indicate that aberrant p63 expression plays a role not only in malignant...Continue Reading

References

Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Jan 15, 1983·International Journal of Cancer. Journal International Du Cancer·J GerdesH Stein
Aug 1, 1993·The Journal of Surgical Research·D W FriedmanS B Deak
Oct 20, 1998·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·D A LadinD P Fivenson
Dec 14, 1999·The Journal of Investigative Dermatology·R ParsaH Green
Jan 5, 2000·Oncogene·W G Kaelin
Jan 29, 2000·Journal of Dermatological Science·P TeofoliP Puddu
May 8, 2000·International Journal of Cancer. Journal International Du Cancer·K YamaguchiJ Jen
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·A Yang, F McKeon
Oct 11, 2002·The EMBO Journal·Shelya X ZengHua Lu
Nov 26, 2002·International Journal of Cancer. Journal International Du Cancer·Hai HuMing-Rong Wang

❮ Previous
Next ❯

Citations

Jan 1, 2009·Dermatology Research and Practice·Richard BakerAdriaan Grobbelaar
Feb 16, 2007·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Rajiv Y ChandawarkarPeter J Deckers
Oct 28, 2008·Burns : Journal of the International Society for Burn Injuries·Guang-Qing Wang, Zhao-Fan Xia
Apr 5, 2008·Journal of the American College of Surgeons·Paris D ButlerGeorge P Yang
Apr 25, 2006·Journal of Cutaneous Pathology·Sema BircanSirin Baspinar
Sep 24, 2015·BMC Research Notes·Bruna De FeliceMassimo Nacca
Dec 12, 2007·American Journal of Surgery·Paris D ButlerGeorge P Yang
May 19, 2005·Annals of Plastic Surgery·Bishara S AtiyehShady N Hayek
Nov 29, 2007·Journal of Cutaneous Medicine and Surgery·Cagdas OzdolCetin Erol
Feb 19, 2009·Molecular and Cellular Biochemistry·Bruna De FeliceRobert R Wilson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Dermatological Science
Aya TanakaSachiko Miyagawa
Proceedings of the National Academy of Sciences of the United States of America
S B RussellJ S Trupin
Proceedings of the National Academy of Sciences of the United States of America
G PellegriniM De Luca
Journal of Dermatological Science
Maranke I Koster, Dennis R Roop
© 2022 Meta ULC. All rights reserved