Molecular characterization and expression of three GnRH forms mRNA during gonad sex-change process, and effect of GnRHa on GTH subunits mRNA in the protandrous black porgy (Acanthopagrus schlegeli)

General and Comparative Endocrinology
Kwang Wook AnCheol Young Choi

Abstract

Gonadotropin-releasing hormone (GnRH) plays a pivotal role in control of reproduction and gonadal maturation in teleost fish. To investigate the action GnRH in black porgy (Acanthopagrus schlegeli), we examined the mRNA expression of GTH subunits (GTHalpha, FSHbeta, and LHbeta) in the pituitary as well as plasma estradiol-17beta (E(2)) level following treatment with a GnRH analog (GnRHa) in immature fish. The expression levels of GTH subunits mRNA and plasma E(2) level were increased after GnRHa injection. We were also able to identify three GnRH forms: salmon GnRH (sGnRH), seabream GnRH (sbGnRH) and chicken GnRH-II (cGnRH-II) by cDNA cloning in the ovary of the black porgy. Black porgy gonadal development is divided into seven stages, involving sex change from male to female (immature testis, mature testis, testicular portion of mostly testis, ovarian portion of mostly testis, testicular portion of mostly ovary, ovarian portion of mostly ovary, and mature ovary). In the present study, we investigated the expression pattern of three GnRH molecular forms in the black porgy gonads at different stages of gonadal development by quantitative polymerase chain reaction (QPCR). The mRNA expressions of sGnRH, sbGnRH and cGnRH-II were fo...Continue Reading

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Citations

Mar 9, 2012·Journal of Molecular Neuroscience : MN·Gal Levy, Gad Degani
May 9, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Na Na KimCheol Young Choi
Nov 1, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Na Na KimCheol Young Choi
Mar 30, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Kwang Wook AnCheol Young Choi
Nov 18, 2009·General and Comparative Endocrinology·Guan-Chung WuChing-Fong Chang
Aug 19, 2009·General and Comparative Endocrinology·B Levavi-SivanJ J Lareyre
Jun 30, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·G LevyG Degani
Jun 6, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Hyun Suk ShinCheol Young Choi
Dec 9, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Kwang Wook AnCheol Young Choi
Dec 17, 2021·Journal of Neuroendocrinology·Yonathan ZoharMatan Golan

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