Molecular Characterization and Expression of OfJNK and Ofp38 in Ostrinia furnacalis (Guenée) Under Different Environmental Stressors

Frontiers in Physiology
Li SuChang-Yu Zhang

Abstract

Ostrinia furnacalis, an important pest of corn, has substantial detrimental effects on corn production. The mitogen-activated protein kinase (MAPK) signaling pathway plays a pivotal role in an insect's resistance to environmental stress. The expression levels of JNK and p38 have been well recorded in several insects under different environmental stressors, at different developmental stages, and in various tissue types; however, there is limited information on JNK and p38 in agricultural insects. To clarify the mechanism whereby O. furnacalis responds to environmental stress, we cloned JNK and p38 from O. furnacalis and subsequently named them OfJNK and Ofp38, respectively. Further, we examined the expression levels of OfJNK and Ofp38 under different environmental stressors. In this study, we obtained full-length sequences of OfJNK and Ofp38, and RT-qPCR results showed that these genes were expressed at all developmental stages, in various tissues (head, chest, abdomen, leg, wing, antennae, compound eye, midgut, and ovary) and under different environmental stressors (4°C and ultraviolet A treatment for 0, 30, 60, 90, and 120 min). The expression levels of OfJNK and Ofp38 were relatively higher in eggs and 3-day-old adult females...Continue Reading

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Citations

Apr 13, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Zeyang SunChuncai Yan

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Datasets Mentioned

BETA
MK779706
MK779707

Methods Mentioned

BETA
environmental stresses
electrophoresis
PCR
environmental stress

Software Mentioned

Basic Local Alignment Search Tool
DNAMAN
SMART
MEGA
Primer Premier
ProtParam
NetPhos

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