Molecular characterization and expression of heparan-sulfate 6-sulfotransferase. Complete cDNA cloning in human and partial cloning in Chinese hamster ovary cells.

The Journal of Biological Chemistry
Hiroko HabuchiKoji Kimata

Abstract

Heparan-sulfate 6-sulfotransferase (HS6ST) catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 6 of the N-sulfoglucosamine residue of heparan sulfate. The enzyme was purified to apparent homogeneity from the serum-free culture medium of Chinese hamster ovary (CHO) cells (Habuchi, H., Habuchi, O., and Kimata, K. (1995) J. Biol Chem. 270, 4172-4179). From the amino acid sequence data of the purified enzyme, degenerate oligonucleotides were designed and used as primers for the reverse transcriptase-polymerase chain reaction using poly(A)+ RNA from CHO cells as a template. The amplified cDNA fragment was then used as a probe to screen a cDNA library of CHO cells. The cDNA clone thus obtained encoded a partial peptide sequence composed of 236 amino acid residues that included the sequences of six peptides obtained after endoproteinase digestion of the purified enzyme. This cDNA clone was applied to the screening of a human fetal brain cDNA library by cross-hybridization. The isolated cDNA clones contained a whole open reading frame that predicts a type II transmembrane protein composed of 401 amino acid residues. No significant amino acid sequence identity to any other proteins, including heparan...Continue Reading

References

Jan 1, 1992·Annual Review of Cell Biology·M BernfieldE J Lose
Oct 1, 1992·Current Opinion in Cell Biology·T N WightE E Qwarnström
Jan 1, 1991·Annual Review of Biochemistry·L Kjellén, U Lindahl
Feb 1, 1989·The Journal of Cell Biology·M Kozak
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Dec 8, 1995·The Journal of Biological Chemistry·S AshikariK Kimata
Aug 4, 1995·The Journal of Biological Chemistry·M FukutaO Habuchi
Oct 1, 1993·Current Opinion in Cell Biology·A C Rapraeger
Aug 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G David
Apr 30, 1996·Annals of the New York Academy of Sciences·V Luu-TheF Labrie
Jul 26, 1996·The Journal of Biological Chemistry·X Bai, J D Esko
Sep 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M SalmivirtaU Lindahl
Oct 25, 1996·The Journal of Biological Chemistry·J LiuR D Rosenberg
Feb 1, 1996·Annals of Medicine·P Inki, M Jalkanen
Jan 1, 1997·Infection and Immunity·K S Rostand, J D Esko
May 23, 1997·The Journal of Biological Chemistry·M KobayashiK Kimata
Sep 12, 1997·The Journal of Biological Chemistry·X BaiJ D Esko
Jan 24, 1998·The Journal of Biological Chemistry·M FukutaO Habuchi

❮ Previous
Next ❯

Citations

Sep 28, 2000·Biochemical and Biophysical Research Communications·M HernaizR J Linhardt
Feb 18, 2004·The Journal of Laboratory and Clinical Medicine·Kenji NakayamaYasuhiro Natori
Apr 1, 2000·Biochimica Et Biophysica Acta·O Habuchi
Nov 6, 2002·Biochimica Et Biophysica Acta·Dorota A Bulik, Phillips W Robbins
Nov 6, 2002·Biochimica Et Biophysica Acta·Hiroshi Nakato, Koji Kimata
Aug 30, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·L A FranssonA Oldberg
Aug 20, 2009·European Journal of Human Genetics : EJHG·Xenia KaidonisSharon Byers
Aug 2, 2011·Nature Biotechnology·Xun XuJun Wang
Aug 2, 2001·Glycobiology·A Kobata
Feb 12, 2010·BMC Biotechnology·Zhengliang L WuWeiping Jiang
Aug 16, 2014·BioMed Research International·Shuji MizumotoKazuyuki Sugahara
Mar 1, 2007·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Michele M SkopecM Denise Dearing
Nov 1, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Adam B Cadwallader, H Joseph Yost
Jan 16, 1999·Chemistry & Biology·K G Bowman, C R Bertozzi
Jan 29, 2014·Cytotechnology·Payel DattaSusan T Sharfstein
Nov 23, 2006·Methods in Enzymology·Hiroko HabuchiTakashi Muramatsu
Jan 7, 2004·The Journal of Biological Chemistry·Satoko Ashikari-HadaKoji Kimata
Oct 20, 1999·FEBS Letters·S YamadaK Sugahara
Jul 2, 2005·Journal of Cellular Physiology·Guido J JenniskensToin H van Kuppevelt
Oct 10, 2002·Endocrine Reviews·Charles A Strott
Mar 17, 2007·The Journal of Biological Chemistry·Naoko NagaiKoji Kimata
Nov 29, 2001·The Journal of Cell Biology·B L AllenA C Rapraeger
Mar 29, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zhengliang L WuRobert D Rosenberg
Dec 23, 1999·The Journal of Biological Chemistry·J LiuR D Rosenberg
Mar 18, 2000·The Journal of Biological Chemistry·S YamauchiO Habuchi
Sep 17, 1998·The Journal of Biological Chemistry·U LindahlL Kjellén
Jun 5, 2003·The Journal of Biological Chemistry·Robert J BinkDanica Zivkovic

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.