PMID: 20135848Feb 6, 2010Paper

Molecular characterization and expression pattern of two zona pellucida genes in half-smooth tongue sole (Cynoglossus semilaevis)

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Yeying SunChunmei Li

Abstract

Zona pellucida (ZP) proteins are glycoproteins in fish chorion that are encoded by multiple gene families, mainly including zp1, zp2, and zp3. In the present study, we cloned two zp genes of half-smooth tongue sole, Cynoglossus semilaevis, using reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The two genes were demonstrated to belong to zp3 isoforms by utilizing BLAST, alignment of amino acid sequence, and phylogenetic analysis. The two genes were named C. semilaevis zp3a and C. semilaevis zp3b. C. semilaevis zp3a encoded 519 amino acid protein, including a signal peptide and a ZP domain with 257 amino acids, which was 62% identical to the medaka ZPC5. Incontrast, C. semilaevis zp3b encoded a 313 amino acid protein, including a signal peptide and a ZP domain with 233 amino acids, which was 63% identical to the medaka (Oryzias latipes) ZPC1. Alignment analysis showed that the ZP domain of the two ZP proteins contained eight conserved cysteines. RT-PCR indicated that the C. semilaevis zp3a was highly expressed in the ovary and kidney of females, and weakly expressed in female muscle and spleen. The C. semilaevis zp3b mRNA was expressed in several tissues of females,including a...Continue Reading

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Citations

Jan 14, 2011·Fish Physiology and Biochemistry·Ksenia J GrohMarc J-F Suter
Apr 23, 2013·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Cong YuanZaizhao Wang
Oct 15, 2013·Molecular Ecology·Giacomo Bernardi
Jun 14, 2016·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Zhi-Jian WangYao-Guang Zhang
Jan 8, 2018·Fish Physiology and Biochemistry·Tianli WuDeshou Wang

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