Molecular characterization and functional analysis of three pathogenesis-related cytochrome P450 genes from Bursaphelenchus xylophilus (Tylenchida: Aphelenchoidoidea)

International Journal of Molecular Sciences
Xiao-Lu XuLin Huang

Abstract

Bursaphelenchus xylophilus, the causal agent of pine wilt disease, causes huge economic losses in pine forests. The high expression of cytochrome P450 genes in B. xylophilus during infection in P. thunbergii indicated that these genes had a certain relationship with the pathogenic process of B. xylophilus. Thus, we attempted to identify the molecular characterization and functions of cytochrome P450 genes in B. xylophilus. In this study, full-length cDNA of three cytochrome P450 genes, BxCYP33C9, BxCYP33C4 and BxCYP33D3 were first cloned from B. xylophilus using 3' and 5' RACE PCR amplification. Sequence analysis showed that all of them contained a highly-conserved cytochrome P450 domain. The characteristics of the three putative proteins were analyzed with bioinformatic methods. RNA interference (RNAi) was used to assess the functions of BxCYP33C9, BxCYP33C4 and BxCYP33D3. The results revealed that these cytochrome P450 genes were likely to be associated with the vitality, dispersal ability, reproduction, pathogenicity and pesticide metabolism of B. xylophilus. This discovery confirmed the molecular characterization and functions of three cytochrome P450 genes from B. xylophilus and provided fundamental information in elucidat...Continue Reading

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Citations

Oct 24, 2018·Molecular Plant-microbe Interactions : MPMI·Long-Jiao HuJian-Ren Ye
Apr 11, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Wei ZhangJianghua Sun

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Datasets Mentioned

BETA
AAF58186.1

Methods Mentioned

BETA
PCR
chips
electrophoresis
optical microscopy

Software Mentioned

DNAMAN
ABI Prism 7500
Excel
MEGA
Blastp
SignalP
TMHMM
SPSS Statistics

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