Molecular characterization and functional analysis of the Schistosoma mekongi Ca2+ -dependent cysteine protease (calpain)

Parasites & Vectors
Salisa ChaimonPoom Adisakwattana

Abstract

Schistosoma mekongi, which causes schistosomiasis in humans, is an important public health issue in Southeast Asia. Treatment with praziquantel is the primary method of control but emergence of praziquantel resistance requires the development of alternative drugs and vaccines. Calcium-dependent cysteine protease (calpain) is a novel vaccine candidate that has been studied in S. mansoni, S. japonicum, and protozoans including malaria, leishmania and trypanosomes. However, limited information is available on the properties and functions of calpain in other Schistosoma spp., including S. mekongi. In this study, we functionally characterized calpain 1 of S. mekongi (SmeCalp1). Calpain 1 of S. mekongi was obtained from transcriptomic analysis of S. mekongi; it had the highest expression level of all isoforms tested and was predominantly expressed in the adult male. SmeCalp1 cDNA is 2274 bp long and encodes 758 amino acids, with 85% to 90% homology with calpains in other Schistosoma species. Recombinant SmeCalp1 (rSmeCalp1), with a molecular weight of approximately 86.7 kDa, was expressed in bacteria and stimulated a marked antibody response in mice. Native SmeCalp1 was detected in crude worm extract and excretory-secretory product, ...Continue Reading

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Datasets Mentioned

BETA
MK610444

Methods Mentioned

BETA
electron microscopy
PCR
electrophoresis
Protein Assay
transmission electron microscopy
ELISA
myristoylation

Software Mentioned

SecretomeP
SwissModel
NetNGlyc
SABLE
Polyview
iCn3D
SignalP
BLASTP
NetOGlyc
MEGA7

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