Molecular characterization and gene expression modulation of the alternative oxidase in a scuticociliate parasite by hypoxia and mitochondrial respiration inhibitors.

Scientific Reports
Iría FolgueiraJosé Manuel Leiro

Abstract

Philasterides dicentrarchi is a marine benthic microaerophilic scuticociliate and an opportunistic endoparasite that can infect and cause high mortalities in cultured turbot (Scophthalmus maximus). In addition to a cytochrome pathway (CP), the ciliate can use a cyanide-insensitive respiratory pathway, which indicates the existence of an alternative oxidase (AOX) in the mitochondrion. Although AOX activity has been described in P. dicentrarchi, based on functional assay results, genetic evidence of the presence of AOX in the ciliate has not previously been reported. In this study, we conducted genomic and transcriptomic analysis of the ciliate and identified the AOX gene and its corresponding mRNA. The AOX gene (size 1,106 bp) contains four exons and three introns that generate an open reading frame of 915 bp and a protein with a predicted molecular weight of 35.6 kDa. The amino acid (aa) sequence of the AOX includes an import signal peptide targeting the mitochondria and the protein is associated with the inner membrane of the mitochondria. Bioinformatic analysis predicted that the peptide is a homodimeric glycoprotein, although monomeric forms may also appear under native conditions, with EXXH motifs associated with the diiron...Continue Reading

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Citations

Jul 28, 2020·The American Journal of Bioethics : AJOB·Mark P Aulisio, Thomas May
Aug 11, 2020·QJM : Monthly Journal of the Association of Physicians·Julián OlallaUNKNOWN ROBLE group

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Datasets Mentioned

BETA
KF952262
MN193569
MH427340.1
QAR17767.1

Methods Mentioned

BETA
RNA-
Seq
RNA-Seq
PCR
electrophoresis
fluorescence microscopy

Software Mentioned

Phobius
MRBAYES
MEGAX
OGLCNACSCAN
BLASTx
MODEL
LOCTREE3
Swiss
TULIP
TMHMM

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