Abstract
Stubby root nematodes (SRN) are important plant parasites infecting many crops and widely distributed in many regions of the United States. SRN transmit Tobacco rattle virus, which causes potato corky ringspot disease, thereby having a significant economic impact on the potato industry. In 2015 to 2017, 184 soil samples and 16 nematode suspensions from North Dakota, Minnesota, Idaho, Oregon, Washington, South Carolina, North Carolina, and Florida were assayed for the presence of SRN. SRN were found in 106 soil samples with population densities of 10 to 320 SRN per 200 g of soil and in eight of the nematode suspensions. Sequencing of ribosomal DNA (rDNA) or species-specific polymerase chain reaction assays revealed the presence of four SRN species, including Paratrichodorus allius, P. minor, P. porosus, and Trichodorus obtusus. Accordingly, their rDNA sequences were characterized by analyzing D2-D3 of 28S rDNA, 18S rDNA, and internal transcribed spacer (ITS) rDNA obtained in this study and retrieved from GenBank. Both intra- and interspecies variations were higher in ITS rDNA than 18S rDNA and D2-D3 of 28S rDNA. Based on phylogenetic analysis, the four SRN species formed a monophyletic group, with P. allius more closely related ...Continue Reading
References
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Feb 28, 1998·Nucleic Acids Research·J D ThompsonD G Higgins
Mar 24, 1998·Nature·M L BlaxterW K Thomas
Apr 26, 2002·Molecular Ecology·Robin FloydMark Blaxter
Oct 11, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Mark BlaxterEyualem Abebe
Mar 28, 2006·Molecular and Cellular Probes·R HolevaV C Blok
Apr 6, 2006·Endoscopy·S UhlenL Michaud
Jun 23, 2006·Molecular Biology and Evolution·Martijn HoltermanJohannes Helder
Apr 21, 2007·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·Y A CarpentierW J Malaisse
Jun 3, 2008·Molecular Phylogenetics and Evolution·Sergei A SubbotinJames G Baldwin
Jun 13, 2008·Molecular Phylogenetics and Evolution·Martijn HoltermanJohannes Helder
Mar 4, 2014·Applied and Environmental Microbiology·Fei WangChao-Xi Luo
Nov 26, 2015·PloS One·Weimin YeJames Kerns
Jan 21, 2016·BMC Plant Biology·Jenny HagenbladMatti W Leino
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Aug 6, 2017·Molecular Phylogenetics and Evolution·Toon JanssenWim Bert
Feb 1, 2015·Plant Disease·W YeJ Kerns
Aug 1, 2014·Plant Disease·H D Lopez-NicoraT L Niblack
Dec 1, 2005·Plant Disease·E Riga, R Neilson