Molecular characterization and immunodiagnostics of Dicrocoelium dendriticum species isolated from sheep of north-west Himalayan region.

Journal of Helminthology
J S DarB A Ganai

Abstract

Despite its extensive presence among grazing ruminants, dicrocoeliosis, also known as 'small liver fluke' disease, is poorly known and often underestimated by researchers and practitioners in many countries. The accurate identification and prepatent diagnosis of Dicrocoelium dendriticum infection is an essential prerequisite for its prevention and control. In the present study, the morphologically identified specimens isolated from the bile ducts of sheep (Ovis aries) were validated through molecular data. The sequence analysis of the second internal transcribed spacer (ITS-2) of our isolates showed a high degree of similarity with D. dendriticum using the BLAST function of the National Center for Biotechnology Information (NCBI). The phylogenetic analysis of our isolates showed a close relationship with previously described D. dendriticum isolates from different countries. The antigenic profiles of somatic and excretory/secretory (E/S) antigens of D. dendriticum were revealed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting using sera from sheep naturally infected with D. dendriticum. By SDS-PAGE, 16 distinct bands were revealed from crude somatic fraction. Immunoblotting analysis of ...Continue Reading

References

Jul 1, 1991·Veterinary Parasitology·Y TheodoridisC A Himonas
Jun 15, 1989·Molecular and Biochemical Parasitology·J P Dalton, M Heffernan
Dec 1, 1987·Veterinary Immunology and Immunopathology·R M ParkhouseW Harnett
Jan 31, 1997·The Journal of Biological Chemistry·A K RashidL Moens
Dec 13, 2002·Molecular and Cellular Probes·A MarcillaS Mas-Coma
Dec 19, 2002·Trends in Parasitology·Domenico Otranto, Donato Traversa
Feb 1, 2005·Journal of Autism and Developmental Disorders·Wendy L StoneStacie L Pozdol
Sep 17, 2005·Veterinary Parasitology·B Revilla-NuínC González-Lanza
Oct 19, 2005·Advances in Parasitology·Matthew J Nolan, Thomas H Cribb
May 9, 2006·The Journal of Veterinary Medical Science·Kensuke TairaYoshikazu Adachi
Feb 26, 2009·International Journal of Environmental Health Research·Eduardo de MasiMaria Tereza Pepe Razzolini
Sep 3, 2009·Experimental Parasitology·Panat AnuracpreedaPrasert Sobhon
Jan 29, 2010·Parasitology Research·Madoka Ichikawa, Tadashi Itagaki
Jun 28, 2011·Parasitology International·Si-Yang HuangXing-Quan Zhu
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.